Dr Yung-Yao Lin

Yung-Yao Lin

Senior Lecturer

Centre for Genomics and Child Health, Blizard Institute
Faculty of Medicine and Dentistry, Queen Mary University of London
www.qmul.ac.uk/blizard/all-staff/profiles/yung-yao-lin.html
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Research

Human induced pluripotent stem cells, CRISPR genome editing, Neuromuscular disorders, Disease modelling, Regenerative medicine, Tissue engineering

Underpinning Bioengineering, Cardiovascular Models, Infectious Disease and Inflammation Models, Musculoskeletal Models, Neuronal Models

Interests

Research in the Lin group is focused on muscular dystrophy and mechanisms regulating muscle regeneration. His work has brought novel insights into the pathological mechanisms underlying some types of muscular dystrophy. The Lin group has established essential expertise in generation and differentiation of human induced pluripotent stem cells (iPSCs) and CRISPR-mediated genome manipulation technology. We are interested in integrating human iPSC and Organ-on-a-Chip technologies to enable neuromuscular research in health and disease, as well as drug discovery.

Publications

Publications of specific relevance to Predictive in vitro Models

2023

Harley P, Paredes-Redondo A, Grenci G, Viasnoff V, Lin Y-Y and Lieberam I (2023). 3D Compartmentalised Human Pluripotent Stem Cell–derived Neuromuscular Co-cultures. Bio-Protocol  vol. 13, (5) e4624-e4624. 10.21769/bioprotoc.4624

2022

Morera C, Kim J, Paredes-Redondo A, Nobles M, Rybin D, Moccia R, Kowala A, Meng J, Garren S, Liu P, Morgan JE, Muntoni F, Christoforou N, Owens J, Tinker A and Lin Y-Y (2022). CRISPR-mediated correction of skeletal muscle Ca2+ handling in a novel DMD patient-derived pluripotent stem cell model. Neuromuscular Disorders  10.1016/j.nmd.2022.10.007
Chesshyre M, Ridout D, Hashimoto Y, Ookubo Y, Torelli S, Maresh K, Ricotti V, Abbott L, Gupta VA, Main M, Ferrari G, Kowala A, Lin Y, Tedesco FS, Scoto M, Baranello G, Manzur A, Aoki Y and Muntoni F (2022). Investigating the role of dystrophin isoform deficiency in motor function in Duchenne muscular dystrophy. Wiley  Journal of Cachexia Sarcopenia and Muscle  vol. 13, (2) 1360-1372. 10.1002/jcsm.12914

2021

Marino S (2021). Comparative epigenetic analysis of tumour initiating cells and syngeneic EPSC‐derived neural stem cells in glioblastoma. Nature Research (Part of Springer Nature)  Nature Communications  10.1038/s41467-021-26297-6
Paredes-Redondo A, Harley P, Maniati E, Ryan D, Louzada S, Meng J, Kowala A, Fu B, Yang F, Liu P, Marino S, Pourquié O, Muntoni F, Wang J, Lieberam I and Lin YY (2021). Optogenetic modeling of human neuromuscular circuits in Duchenne muscular dystrophy with CRISPR and pharmacological corrections. Science Advances  vol. 7, (37) 10.1126/sciadv.abi8787

2019

Kim J, Lana B, Torelli S, Ryan D, Catapano F, Ala P, Luft C, Stevens E, Konstantinidis E, Louzada S, Fu B, Paredes‐Redondo A, Chan AE, Yang F, Stemple DL, Liu P, Ketteler R, Selwood DL, Muntoni F and Lin Y (2019). A new patient‐derived iPSC model for dystroglycanopathies validates a compound that increases glycosylation of α‐dystroglycan. Springer Nature  Embo Reports  vol. 20, (11) 10.15252/embr.201947967
Paredes‐Redondo A and Lin Y (2019). Human Induced Pluripotent Stem Cells: Challenges and Opportunities in Developing New Therapies for Muscular Dystrophies. Encyclopedia of Life Sciences  Wiley  10.1002/9780470015902.a0028371

2016

Bevan N, Bevan L, Brown S, van Bokhoven H, Campbell KP, Cirak S, Cisneros B, Colognato H, Hohenester E, Lefeber D, Liljedahl M, Lu Q, Seta N, Shcherbata H, Straub V, Winder S and LIN Y (2016). 220th ENMC workshop: Dystroglycan and the dystroglycanopathies Naarden, The Netherlands, 27–29 May 2016. 10.1016/j.nmd.2016.12.010
Praissman JL, Willer T, Sheikh MO, Toi A, Chitayat D, Lin Y-Y, Lee H, Stalnaker SH, Wang S, Prabhakar PK, Nelson SF, Stemple DL, Moore SA, Moremen KW, Campbell KP and Wells L (2016). The functional O-mannose glycan on α-dystroglycan contains a phospho-ribitol primed for matriglycan addition. Elife  vol. 5, 10.7554/eLife.14473

2013

Carss KJ, Stevens E, Foley AR, Cirak S, Riemersma M, Torelli S, Hoischen A, Willer T, van Scherpenzeel M, Moore SA, Messina S, Bertini E, Bönnemann CG, Abdenur JE, Grosmann CM, Kesari A, Punetha J, Quinlivan R, Waddell LB, Young HK, Wraige E, Yau S, Brodd L, Feng L, Sewry C, MacArthur DG, North KN, Hoffman E, Stemple DL and Hurles ME (2013). Mutations in GDP-mannose pyrophosphorylase B cause congenital and limb-girdle muscular dystrophies associated with hypoglycosylation of α-dystroglycan. Am J Hum Genet  vol. 93, (1) 29-41. 10.1016/j.ajhg.2013.05.009
Carss KJ, Stevens E, Foley AR, Cirak S, Riemersma M, Torelli S, Hoischen A, Willer T, Van Scherpenzeel M, Moore SA, Messina S, Bertini E, Bönnemann CG, Abdenur JE, Grosmann CM, Kesari A, Punetha J, Quinlivan R, Waddell LB, Young HK, Wraige E, Yau S, Brodd L, Feng L, Sewry C, MacArthur DG, North KN, Hoffman E, Stemple DL and Hurles ME (2013). Mutations in GDP-mannose pyrophosphorylase B cause congenital and limb-girdle muscular dystrophies associated with hypoglycosylation of α-dystroglycan. American Journal of Human Genetics  vol. 93, (1) 29-41. 10.1016/j.ajhg.2013.05.009
Stevens E, Carss KJ, Cirak S, Foley R, Torelli S, Willer T, Tambunan DE, Yau S, Brodd L, Sewry CA, Feng L, Haliloglu G, Orhan D, Dobyns WB, Enns GM, Manning M, Krause A, Salih MA, Walsh CA, Hurles M, Campbell KP, Manzini MC, Stemple D, Lin Y-Y and Muntoni F (2013). Mutations in B3GALNT2 Cause Congenital Muscular Dystrophy and Hypoglycosylation of α-Dystroglycan. American Journal of Human Genetics  vol. 92, (3) 354-365. 10.1016/j.ajhg.2013.01.016
Buysse K, Riemersma M, Powell G, van Reeuwijk J, Chitayat D, Roscioli T, Kamsteeg E-J, van den Elzen C, van Beusekom E, Blaser S, Babul-Hirji R, Halliday W, Wright GJ, Stemple DL, Lin Y-Y, Lefeber DJ and van Bokhoven H (2013). Missense mutations in-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1) cause WalkerWarburg syndrome. Human Molecular Genetics  vol. 22, (9) 1746-1754. 10.1093/hmg/ddt021

2012

Lin Y-Y (2012). Muscle diseases in the zebrafish. Neuromuscul Disord  vol. 22, (8) 673-684. 10.1016/j.nmd.2012.04.007
Roscioli T, Kamsteeg EJ, Buysse K, Maystadt I, Van Reeuwijk J, Van Den Elzen C, Van Beusekom E, Riemersma M, Pfundt R, Vissers LELM, Schraders M, Altunoglu U, Buckley MF, Brunner HG, Grisart B, Zhou H, Veltman JA, Gilissen C, Mancini GMS, Delrée P, Willemsen MA, Ramadža DP, Chitayat D, Bennett C, Sheridan E, Peeters EAJ, Tan-Sindhunata GMB, De Die-Smulders CE, Devriendt K and Kayserili H (2012). Mutations in ISPD cause Walker-Warburg syndrome and defective glycosylation of α-dystroglycan. Nature Genetics  vol. 44, (5) 581-585. 10.1038/ng.2253

2011

Lin Y-Y, White RJ, Torelli S, Cirak S, Muntoni F and Stemple DL (2011). Zebrafish Fukutin family proteins link the unfolded protein response with dystroglycanopathies. Hum Mol Genet  vol. 20, (9) 1763-1775. 10.1093/hmg/ddr059

2009

Lin Y-Y and Gubb D (2009). Molecular dissection of Drosophila Prickle isoforms distinguishes their essential and overlapping roles in planar cell polarity. Dev Biol  vol. 325, (2) 386-399. 10.1016/j.ydbio.2008.10.042

Grants

Grants of specific relevance to Predictive in vitro Models
Engineering Circadian Biology into Induced Pluripotent Stem Cell Organ-on-a-Chip Models
Lee DA, Heywood HK, Lin Y-Y and O'Neill JS
£201,874 National Centre for the Replacement Refinement and Reduction of Animals in Research (01-02-2023 - 30-08-2024)
Summary

3D bio-printing human pluripotent stem cell-derived skeletal muscle constructs for disease modelling and drug discovery
Lin Y-Y and Moreno Sierra N
National Centre for the Replacement Refinement and Reduction of Animals in Research (09-11-2020 - 08-11-2023)
Summary
3D bio-printing human pluripotent stem cell-derived skeletal muscle constructs for disease modelling and drug discovery
Lin Y-Y
£91,606 National Centre for the Replacement Refinement and Reduction of Animals in Research (01-11-2020 - 31-10-2023)
Summary
Assessing regenerative potential of myogenic progenitors derived from CRISPR-corrected human iPSCs for treating muscular dystrophy
Lin Y
£250,401 Barts Charity (07-01-2019 - 06-01-2022)
Engineering a human microphysiological platform for modelling soft tissue injury and regeneration
Lin Y-Y
£100,000 National Centre for the Replacement, Refinement and Reduction of Animals in Research (04-01-2021 - 11-08-2021)
CRISPR/Cas9 Engineered Human IPSC Models for Muscular Dystrophy
Lin Y-Y
£490,000 Pfizer (13-11-2017 - 31-03-2021)
A microphysiological human 3D neuromuscular platform for modelling DMD and assessing potential therapeutics
Lin Y-Y
EUR25,000 Duchenne Parent Project (01-02-2020 - 01-02-2021)
Assessing Regenerative Potential of Myogenic Progenitors Derived frm CRISPR-corrected Human IPSCs
Lin Y
£25,320 Action Duchenne (01-10-2018 - 30-09-2019)
Developing an isogenic iPSC derived model to elucidate the role of dystrophin function in the central nervous system
Lin Y
£15,000 Newlife Foundation for Disabled Children (01-09-2015 - 31-08-2016)
Isogenic control and patient-specific induced pluripotent stem cells as a model for studying neural pathogenesis in muscular dystrophy
Lin Y
£15,000 Royal Society (20-06-2014 - 19-06-2015)