Dr Adrian Biddle
DHT Lecturer in Animal Replacement Science
Organizer for the Organ-on-a-Ship e-Symposia
Centre for Cell Biology & Cutaneous Research, Blizard Institute
Faculty of Medicine and Dentistry, Queen Mary University of London
Faculty of Medicine and Dentistry, Queen Mary University of London
Research
cancer stem cells, tumour metastasis, 3D tumour invasion, metastasis on a chip, organ-on-a-chip, machine learning
Interests
Cancer stem cells drive tumour metastasis and therapeutic resistance. Understanding the underlying mechanisms will be crucial if we are to deliver better treatments for cancer, and we model this process using human cancer cell lines in advanced 3D invasion and microfluidic metastasis systems. We also use human tissue archives, which provide a wealth of information on tumour behaviour in the human body, including data on the clinical course of disease. We have developed new ways of obtaining greater information from these tumour specimens, including simultaneous measurement of multiple cancer stem cell markers and a machine learning approach for prognostic prediction.Publications
2022
Marles H and Biddle A (2022). Cancer stem cell plasticity and its implications in the development of new clinical approaches for oral squamous cell carcinoma. Biochemical Pharmacology vol. 204, 10.1016/j.bcp.2022.115212Scemama A, Lunetto S and Biddle A (2022). Highlight: microfluidic devices for cancer metastasis studies. In Vitro Models vol. 1, (6) 399-403. 10.1007/s44164-022-00023-ySahoo S, Ashraf B, Duddu AS, Biddle A and Jolly MK (2022). Interconnected high-dimensional landscapes of epithelial–mesenchymal plasticity and stemness in cancer. Clinical and Experimental Metastasis vol. 39, (2) 279-290. 10.1007/s10585-021-10139-22020
Custódio M, Biddle A and Tavassoli M (2020). Portrait of a CAF: The story of cancer-associated fibroblasts in head and neck cancer. Oral Oncology vol. 110, 10.1016/j.oraloncology.2020.1049722019
Jolly MK, Somarelli JA, Sheth M, Biddle A, Tripathi SC, Armstrong AJ, Hanash SM, Bapat SA, Rangarajan A and Levine H (2019). Hybrid epithelial/mesenchymal phenotypes promote metastasis and therapy resistance across carcinomas. Pharmacology and Therapeutics vol. 194, 161-184. 10.1016/j.pharmthera.2018.09.0072018
MACKENZIE IC, Rodrigues MF, Gammon L, RAHMAN M, BIDDLE A and Nunes FD (2018). Effects of Cetuximab and Erlotinib on the behaviour of cancer stem cells in head and neck squamous cell carcinoma. Impact Journals Oncotarget 10.18632/oncotarget.244162017
O'Brien-Ball C and Biddle A (2017). Reprogramming to developmental plasticity in cancer stem cells. Developmental Biology vol. 430, (2) 266-274. 10.1016/j.ydbio.2017.07.0252016
BIDDLE A, Gammon L, Liang X, Costea DE and Mackenzie IC (2016). Phenotypic Plasticity Determines Cancer Stem Cell Therapeutic Resistance in Oral Squamous Cell Carcinoma. Elsevier: Creative Commons Ebiomedicine 10.1016/j.ebiom.2016.01.0072015
Vig N, Mackenzie IC and Biddle A (2015). Phenotypic plasticity and epithelial-to-mesenchymal transition in the behaviour and therapeutic response of oral squamous cell carcinoma. Journal of Oral Pathology and Medicine vol. 44, (9) 649-655. 10.1111/jop.12306Shigeishi H, Biddle A, Gammon L, Rodini CO, Yamasaki M, Seino S, Sugiyama M, Takechi M and Mackenzie IC (2015). Elevation in 5-FU-induced apoptosis in head and neck cancer stem cells by a combination of CDHP and GSK3β inhibitors. J Oral Pathol Med vol. 44, (3) 201-207. 10.1111/jop.12230Gemenetzidis E, Gammon L, Biddle A, Emich H and Mackenzie IC (2015). Invasive oral cancer stem cells display resistance to ionising radiation. Oncotarget vol. 6, (41) 43964-43977. 10.18632/oncotarget.62682014
Vig N, Biddle A, Gammon L, Emich H and Mackenzie I (2014). Implications of the Cancer Stem Cell (CSC) Paradigm for OMFS Patients and Surgeons. British Journal of Oral and Maxillofacial Surgery vol. 52, (8) 10.1016/j.bjoms.2014.07.0682013
Hufbauer M, Biddle A, Borgogna C, Gariglio M, Doorbar J, Storey A, Pfister H, Mackenzie I and Akguel B (2013). Expression of Betapapillomavirus Oncogenes Increases the Number of Keratinocytes with Stem Cell-Like Properties. Journal of Virology vol. 87, (22) 12158-12165. 10.1128/JVI.01510-13Shigeishi H, Biddle A, Gammon L, Emich H, Rodini CO, Gemenetzidis E, Fazil B, Sugiyama M, Kamata N and Mackenzie IC (2013). Maintenance of stem cell self-renewal in head and neck cancers requires actions of GSK3β influenced by CD44 and RHAMM. Stem Cells vol. 31, (10) 2073-2083. 10.1002/stem.1418Biddle A, Gammon L, Fazil B and Mackenzie IC (2013). CD44 staining of cancer stem-like cells is influenced by down-regulation of CD44 variant isoforms and up-regulation of the standard CD44 isoform in the population of cells that have undergone epithelial-to-mesenchymal transition. Plos One vol. 8, (2) 10.1371/journal.pone.0057314Gammon L, Biddle A, Heywood HK, Johannessen AC and Mackenzie IC (2013). Sub-sets of cancer stem cells differ intrinsically in their patterns of oxygen metabolism. Plos One vol. 8, (4) 10.1371/journal.pone.00624932012
Biddle A and Mackenzie IC (2012). Cancer stem cells and EMT in carcinoma. Cancer Metastasis Rev 10.1007/s10555-012-9345-0VALORANI MG, Montelatici E, Germani A, Biddle A, D'Alessandro D, Strollo R, Patrizi MP, Lazzari L, Nye E and Otto WR (2012). Pre-culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potential. Cell Proliferation vol. 45, (3) 225-238. 10.1111/j.1365-2184.2012.008172011
Biddle A, Liang X, Gammon L, Fazil B, Harper LJ, Emich H, Costea DE and Mackenzie IC (2011). Cancer stem cells in squamous cell carcinoma switch between two distinct phenotypes that are preferentially migratory or proliferative. Cancer Res vol. 71, (15) 5317-5326. 10.1158/0008-5472.CAN-11-1059Gammon L, Biddle A, Fazil B, Harper L and Mackenzie IC (2011). Stem cell characteristics of cell sub-populations in cell lines derived from head and neck cancers of Fanconi anemia patients. J Oral Pathol Med vol. 40, (2) 143-152. 10.1111/j.1600-0714.2010.00972.x2010
Valorani MG, Germani A, Otto WR, Harper L, Biddle A, Khoo CP, Lin WR, Hawa MI, Tropel P, Patrizi MP, Pozzilli P and Alison MR (2010). Hypoxia increases Sca-1/CD44 co-expression in murine mesenchymal stem cells and enhances their adipogenic differentiation potential. Cell Tissue Res vol. 341, (1) 111-120. 10.1007/s00441-010-0982-8Harper LJ, Costea DE, Gammon L, Fazil B, Biddle A and Mackenzie IC (2010). Normal and malignant epithelial cells with stem-like properties have an extended G2 cell cycle phase that is associated with apoptotic resistance. Bmc Cancer vol. 10, 10.1186/1471-2407-10-1662009
Biddle A, Simeoni I and Gurdon JB (2009). Xenopus oocytes reactivate muscle gene transcription in transplanted somatic nuclei independently of myogenic factors. Development vol. 136, (16) 2695-2703. 10.1242/dev.0363272005
Dragnev KH, Petty WJ, Shah S, Biddle A, Desai NB, Memoli V, Rigas JR and Dmitrovsky E (2005). Bexarotene and erlotinib for aerodigestive tract cancer. Journal of Clinical Oncology vol. 23, (34) 8757-8764. 10.1200/JCO.2005.01.9521Petty WJ, Li N, Biddle A, Bounds R, Nitkin C, Ma Y, Dragnev KH, Freemantle SJ and Dmitrovsky E (2005). A novel retinoic acid receptor β isoform and retinoid resistance in lung carcinogenesis. Journal of The National Cancer Institute vol. 97, (22) 1645-1651. 10.1093/jnci/dji3712004
Petty WJ, Bragnev KH, Memoli VA, Ma Y, Desai NB, Biddle A, Davis TH, Nugent WC, Memoli N, Hamilton M, Iwata KK, Rigas JR and Dmitrovsky E (2004). Epidermal growth factor receptor tyrosine kinase inhibition represses cyclin D1 in aerodigestive tract cancers. Clinical Cancer Research vol. 10, (22) 7547-7554. 10.1158/1078-0432.CCR-04-1169Grants
Grants of specific relevance to Predictive in vitro Models
Identifying and manipulating molecular mechanisms controlling cancer stem cell metastatic potential in a human oral cancer model.Biddle AJ and Wang J
£679,533 Medical Research Council (02-08-2021 - 01-08-2024)
A 3Rs approach to tumour metastasis: investigating the role of cancer stem cells in metastasis using an in vitro microfluidic modelBiddle AJ, Scemama A and Gautrot JEE
£90,000 National Centre for the Replacement, Refinement and Reduction of Animals in Research (01-10-2019 - 30-09-2022)
Development of a molecular biomarker test for metastatic cancer stem cells in oral squamous cell carcinomaBiddle AJ and Ambler L
£93,000 Oracle Cancer Trust (02-07-2018 - 31-12-2021)
The Animal Replacement Centre of ExcellencePhilpott MP, Biddle AJ and Youssef G
£1,000,000 Animal Free Research UK (01-08-2016 - 31-07-2021)