Prof Leonardo Guasti

Leonardo Guasti

Professor of Experimental Endocrinology

Centre for Endocrinology, The William Harvey Research Institute
Queen Mary University of London
www.qmul.ac.uk/whri/people/academic-staff/items/guastileonardo.html
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Research

stem cells, adrenal cortex, organoids, replacement cell therapy

Cancer Models

Interests

Expertise in reprogramming strategies to derive adrenocortical cells. Generation of normal and cancerous adrenal organoids from mice and humans.
Funded by MRC (adrenal organoids) and BBSRC (contribution of adrenal stem/progenitor cells to the physiological remodelling of the gland).

Publications

solid heart iconPublications of specific relevance to Predictive in vitro Models

2025

Cover Image, Volume 45, Issue 6
Mariniello K, Pittaway JFH, Altieri B, Borges KS, Hadjidemetriou I, Ribeiro C, Ruiz‐Babot G, Tourigny DS et al.
Cancer Communications, Wiley vol. 45 (6) 
01-06-2025
Defective Nitric oxide pathway signalling - a link between premature birth and altered mini-puberty?
Read J, Delli V, Dunkel L, Santoni F, Prevot V, Guasti L, Chachlaki K, Howard S
Endocrine Abstracts, Bioscientifica 
09-05-2025
Dlk1 is a novel adrenocortical stem/progenitor cell marker that predicts malignancy in adrenocortical carcinoma
Mariniello K, Pittaway JFH, Altieri B, Borges KS, Hadjidemetriou I, Ribeiro C, Ruiz‐Babot G, Tourigny DS et al.
Cancer Communications, Wiley vol. 45 (6), 663-668.  
04-03-2025

2024

The Role of DLK1 Deficiency in Central Precocious Puberty and Association with Metabolic Dysregulation
d'Aniello F, Mariniello K, Al Sayed Y, Bhavsar K, Read JE, Guasti L, Howard SR
Hormone Research in Paediatrics, Karger Publishers vol. 99 (1), 38-48.  
17-10-2024
12408 Efnb2 Controls Pituitary Gland Development By Regulating Proliferation Of Pituitary Stem Cells
Gualtieri A, Gomez-Corral L, Nicholson J, Tan R
Journal of The Endocrine Society, The Endocrine Society vol. 8 (Supplement_1) 
05-10-2024
Efnb2 controls pituitary development by regulating the pituitary stem cell niche
Gualtieri A, Gomez-Corral L, Nicholson J, Tan R, Guasti L, Gaston-Massuet C
Endocrine Abstracts, Bioscientifica 
30-08-2024

2023

Generation of glucocorticoid-producing cells derived from human pluripotent stem cells
Ruiz-Babot G, Eceiza A, Abollo-Jiménez F, Malyukov M, Carlone DL, Borges K, Da Costa AR, Qarin S et al.
Cell Reports Methods, Elsevier vol. 3 (11) 
01-11-2023
Rare variants in the MECP2 gene in girls with central precocious puberty
Read J, Guasti L, Paganoni A, Howard S
Endocrine Abstracts, Bioscientifica 
01-11-2023
Rare variants in the MECP2 gene in girls with central precocious puberty: a translational cohort study
Canton APM, Tinano FR, Guasti L, Montenegro LR, Ryan F, Shears D, de Melo ME, Gomes LG et al.
The Lancet Diabetes & Endocrinology, Elsevier vol. 11 (8), 545-554.  
26-06-2023
The crosstalk between FGF21 and GH leads to weakened GH receptor signaling and IGF1 expression and is associated with growth failure in very preterm infants
Mistry JN, Silvennoinen S, Zaman F, Sävendahl L, Mariniello K, Hall C, Howard SR, Dunkel L et al.
Frontiers in Endocrinology, Frontiers vol. 14 
12-05-2023
Elevated sphingosine-1-phosphate lyase leads to increased metabolism and reduced survival in adrenocortical carcinoma
Williams JL, Smith C, Hall C, Khaled Z, Maharaj A, Kwong R, Pittaway J, Casas J et al.
European Journal of Endocrinology, Oxford University Press (Oup) vol. 188 (1) 
10-01-2023

2022

Locus Coeruleus Neurons’ Firing Pattern Is Regulated by ERG Voltage-Gated K+ Channels
Hasan S, Delicata F, Guasti L, Duranti C, Arcangeli A, Imbrici P, Pessia M
International Journal of Molecular Sciences, Mdpi vol. 23 (23) 
05-12-2022
RF14 | PSAT120 X-Linked Central Precocious Puberty Associated with MECP2 Defects
Canton A, Tinano F, Guasti L, Montenegro L, Argente J, Ryan F, Shears D, Melo ME et al.
Journal of The Endocrine Society, The Endocrine Society vol. 6 (Supplement_1), a643-a644.  
01-11-2022
Delta-like non-canonical Notch ligand 1 (DLK1) - a novel biomarker in adrenocortical carcinoma
Pittaway J, Mariniello K, Altieri B, Sbiera I, Sbiera S, Chung T-T, Abdel-Azziz T, Akker SA et al.
Endocrine Abstracts, Bioscientifica 
27-10-2022
Delta-like non-canonical notch ligand 1 (DLK1)-expressing adrenocortical progenitor cells: role in adrenal turnover, remodeling and tumorigenesis in mice
Mariniello K, Pittaway J, Hadjidemetriou I, Borges K, Doroszko M, Doghman M, Rahman N
Endocrine Abstracts, Bioscientifica 
27-10-2022
Variants in Methyl-CpG-binding protein 2 (MECP2) are associated with X-Linked Central Precocious Puberty
E RJ, Pinheiro-Machado CA, Tinano F, Guasti L, Ribeiro ML, Ryan F, Shears D, Paganoni A et al.
Endocrine Abstracts, Bioscientifica 
27-10-2022
A new recipe for TARTs? One step closer in identifying the origin of testicular adrenal rest tumours
Guasti L, Pittaway JFH
European Journal of Endocrinology, Oxford University Press (Oup) vol. 187 (6), c3-c4.  
26-10-2022
Whole exome sequencing identifies deleterious rare variants in CCDC141 in familial self-limited delayed puberty
T S, G R-B, A D, A M, K M, CP C, MR B, L D et al.
, Bioscientifica 
12-09-2022
DLK1 expressing cells mark a population of progenitor cells in the adrenal cortex and contribute to the zonation of the adrenal gland
Mariniello K, Guasti L
Endocrine Abstracts, Bioscientifica 
07-05-2022
Elevated SGPL1 expression is associated with increased metabolic rate in cells and reduced survival in individuals with adrenocortical carcinoma
Williams J, Smith C, Hall C, Khaled Z, Maharaj A, Kwong R, Pittaway J, Casas J et al.
Endocrine Abstracts, Bioscientifica 
07-05-2022
Novel therapeutic models for adrenal disorders
Guasti L
 
01-01-2022
X-Linked Central Precocious Puberty Associated with MECP2 defects
Canton A, Tinano F, Guasti L, Montenegro L, Ryan F, Shears D, Melo ME, Gomes L et al.
 
01-01-2022

2021

Whole exome sequencing identifies deleterious rare variants in CCDC141 in familial self-limited delayed puberty
Saengkaew T, Ruiz-Babot G, David A, Mancini A, Mariniello K, Cabrera CP, Barnes MR, Dunkel L et al.
Npj Genomic Medicine, Springer Nature vol. 6 (1) 
20-12-2021
Dominant mutations in CCDC141 are found by ehole rxome dequencing to be a common cause of self-limited delayed puberty
Howard S, Saengkaew T, Dunkel L, Guasti L
Endocrine Abstracts, Bioscientifica 
08-11-2021
The role of delta-like non-canonical Notch ligand 1 (DLK1) in cancer.
Pittaway JFH, Lipsos C, Guasti L
Endocrine Related Cancer, Bioscientifica vol. 28 (12), r271-r287.  
15-10-2021
Congenital Adrenal Hyperplasia—Current Insights in Pathophysiology, Diagnostics, and Management
van der Grinten HLC, Speiser PW, Ahmed SF, Arlt W, Auchus RJ, Falhammar H, Flück CE, Guasti L et al.
Endocrine Reviews, The Endocrine Society vol. 43 (1), 91-159.  
07-05-2021
Defects in the GnRH Neuronal Migration factor, CCDC141, Lead to Self-Limited Delayed Puberty
Saengkaew T, Mancini A, Ruiz-Babot G, Cabrera CP, Barnes MR, Dunkel L, Guasti L, Howard S
Journal of The Endocrine Society, The Endocrine Society vol. 5 (Supplement_1), a665-a666.  
03-05-2021
Towards novel treatments for adrenal diseases: Cell- and gene therapy-based approaches
Mariniello K, Guasti L
Molecular and Cellular Endocrinology, Elsevier vol. 524 
14-01-2021

2020

Prevention of anthracycline-induced cardiotoxicity: a systematic review and meta-analysis
Caspani F, Campiotti L, Asteggiano R, Guasti L
Internal and Emergency Medicine, Springer Nature vol. 16 (2), 477-486.  
03-10-2020
LGR4 deficiency results in delayed puberty through impaired Wnt/β-catenin signaling
Mancini A, Howard SR, Marelli F, Cabrera CP, Barnes MR, Sternberg MJE, Leprovots M, Hadjidemetriou I et al.
Jci Insight, American Society For Clinical Investigation vol. 5 (11) 
04-06-2020
SUN-LB43 DLK1 Expressing Cells Contribute to the Zonation of the Adrenal Gland
Mariniello K, Rognoni E
Journal of The Endocrine Society, The Endocrine Society vol. 4 (Supplement_1), sun-lb43.  
08-05-2020

2019

Defects in LGR4 Wnt-[beta]-catenin signalling impair GnRH network development, leading to delayed puberty
Mancini A, Howard SR, Cabrera CP, Barnes MR, David A, Wehkalampi K, Vassert G, Cariboni A et al.
Endocrine Abstracts, Bioscientifica 
11-11-2019
Stem Cells, Self-Renewal, and Lineage Commitment in the Endocrine System
Mariniello K, Ruiz-Babot G, McGaugh EC, Nicholson JG, Gualtieri A, Gaston-Massuet C, Nostro MC, Guasti L
Frontiers in Endocrinology, Frontiers vol. 10 
08-11-2019
Efnb2 controls pituitary gland development by regulating proliferation of the pituitary stem/progenitor cells and EMT within the pituitary stem cell niche
Gualtieri A, Nicholson J, Tan R, Jimenez F, Dattani M, Guasti L, Gaston-Massuet C
Endocrine Abstracts, Bioscientifica 
06-11-2019
HS6ST1 insufficiency causes self-limited delayed puberty in contrast with other GnRH deficiency genes
SR H, R O, A P, V C, A F, G R-B, LA M, CP C et al.
, Bioscientifica 
12-09-2019
EAP1 regulation of GnRH promoter activity is important for human pubertal timing
A M, SR H, CP C, MR B, A D, K W, S H, A L et al.
, Bioscientifica 
12-09-2019
Glucocorticoid replacement therapies: past, present and future
Liew S-Y, Akker SA, Pittaway JFH
Current Opinion in Endocrine and Metabolic Research, Elsevier vol. 8, 152-159.  
04-09-2019
DLK1/PREF1 marks a novel cell population in the human adrenal cortex
Hadjidemetriou I, Mariniello K, Ruiz-Babot G, Pittaway J, Mancini A, Mariannis D, Gomez-Sanchez CE, Parvanta L et al.
The Journal of Steroid Biochemistry and Molecular Biology, Elsevier vol. 193 
29-06-2019
ACTH signalling and adrenal development: lessons from mouse models
Novoselova TV, King PJ, Guasti L, Metherell LA, Clark AJL, Chan LF
Endocrine Connections, Bioscientifica vol. -1 (aop), r122-r130.  
10-06-2019
Isolated glucocorticoid deficiency: Genetic causes and animal models
Maharaj A, Maudhoo A, Chan LF, Novoselova T, Prasad R, Metherell LA, Guasti L
Journal of Steroid Biochemistry and Molecular Biology vol. 189, 73-80.  
01-05-2019
Novel methods in adrenal research: a metabolomics approach
Papathomas TG, Sun N, Chortis V, Taylor AE, Arlt W, Richter S, Eisenhofer G, Ruiz-Babot G et al.
Histochemistry and Cell Biology, Springer Nature vol. 151 (3), 201-216.  
06-02-2019
Pathobiology and genetics of adrenocortical carcinoma
Pittaway JFH
Journal of Molecular Endocrinology, Bioscientifica vol. 62 (2), r105-r119.  
01-02-2019
EAP1 regulation of GnRH promoter activity is important for human pubertal timing
Mancini A, Howard SR, Cabrera CP, Barnes MR, David A, Wehkalampi K, Heger S, Lomniczi A et al.
Human Molecular Genetics, Oxford University Press (Oup) vol. 28 (8), 1357-1368.  
04-01-2019
Defects in the GnRH Neuroendocrine Network Affect the Timing of Puberty
Saengkaew T, Mancini A, Ruiz-Babot G, Cabrera C, Barnes M, Dunkel L, Guasti L, Howard S
 
01-01-2019
Novel interventions to treat adrenal insufficiency
Guasti L
 
01-01-2019
LGR 4-Wnt β-catenin signalling directs GnRH network development, with defects leading to self-limited delayed puberty
Mancini A, Howard SR, Cabrera CP, Barnes MR, David A, Wehkalampi K, Vassart G, Cariboni A et al.
 
01-01-2019

2018

Predicted Benign and Synonymous Variants in CYP11A1 Cause Primary Adrenal Insufficiency Through Missplicing
Maharaj A, Buonocore F, Meimaridou E, Ruiz-Babot G, Guasti L, Peng H-M, Capper CP, Burgos-Tirado N et al.
Journal of The Endocrine Society, The Endocrine Society vol. 3 (1), 201-221.  
30-10-2018
Vitamin D and tyrosine kinase inhibitors in chronic myeloid leukemia
Campiotti L, Sutter MB, Maresca AM, Grandi AM
Internal and Emergency Medicine, Springer Nature vol. 13 (8), 1337-1339.  
25-09-2018
Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells
G R-B, M B, I H, SJ A, DR T, L G, NF T, U S et al.
, Bioscientifica 
11-09-2018
Available oral lipid-lowering agents could bring most high-risk patients to target
De Ferrari GM, Perna GP, Nicosia A, Casu G, Cuccia C, Picco F, Strazzella C, Totaro R et al.
Journal of Cardiovascular Medicine, Wolters Kluwer vol. 19 (9), 485-490.  
01-09-2018
Plasticity of human adipose-derived stem cells - relevance to tissue repair.
Guasti L, New SE, Hadjidemetriou I, Palmiero M
Int J Dev Biol vol. 62 (6-7-8), 431-439.  
21-06-2018
HS6ST1 Insufficiency Causes Self-Limited Delayed Puberty in Contrast With Other GnRH Deficiency Genes
Howard SR, Oleari R, Poliandri A, Chantzara V, Fantin A, Ruiz-Babot G, Metherell LA, Cabrera CP et al.
The Journal of Clinical Endocrinology & Metabolism, The Endocrine Society vol. 103 (9), 3420-3429.  
20-06-2018
Expression of progenitor markers is associated with the functionality of a bioartificial adrenal cortex.
Balyura M, Gelfgat E, Steenblock C, Androutsellis-Theotokis A, Ruiz-Babot G, Guasti L, Ludwig B, Bornstein T et al.
Plos One, Public Library of Science vol. 13 (3), e0194643-e0194643.  
29-03-2018
Role of GnRH Neuronal Migration and Development in Self-Limited Delayed Puberty
Howard S, Oleari R, Mancini A, Poliandri A, Saengkaew T, Guasti L, Cariboni A, Dunkel L
 
01-01-2018
EAP1 Mutations Cause an Impaired Transcriptional Activity on GnRH Promoter That Leads to Self-Limited Delayed Puberty
Mancini A, Howard SR, Cabrera CP, Barnes MR, Heger S, Guasti L, Ojeda S, Dunkel L
 
01-01-2018
Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells
Ruiz-Babot G, Balyura M, Hadjidemetriou I, Ajodha SJ, Taylor DR, Ghataore L, Taylor NF, Schubert U et al.
In Ssrn Electronic Journal 
01-01-2018

2017

Contributions of Function-Altering Variants in Genes Implicated in Pubertal Timing and Body Mass for Self-Limited Delayed Puberty
Howard SR, Guasti L, Poliandri A, David A, Cabrera CP, Barnes MR, Wehkalampi K, O’Rahilly S et al.
The Journal of Clinical Endocrinology & Metabolism, The Endocrine Society vol. 103 (2), 649-659.  
16-11-2017
Patients with self-limited delayed puberty harbour mutations in multiple genes controlling GnRH neuronal development
Howard S, Andre V, Guasti L, Cabrera C, Barnes M, Cariboni A, Dunkel L
Endocrine Abstracts, Bioscientifica 
30-10-2017
Predicted benign and silent SNPs in CYP11A1 cause primary adrenal insufficiency through missplicing
Maharaj A, Buonocore F, Meimaridou E, Ruiz-Babot G, Guasti L, Peng H-M, Capper C, Prasad R et al.
Endocrine Abstracts, Bioscientifica 
20-10-2017
Kisspeptin is a novel regulator of human fetal adrenocortical development and function – a finding with important implications for the human feto-placental unit
Katugampola H, King PJ, Chatterjee S, Meso M, Duncan AJ, Achermann JC, Guasti L, Ghataore L et al.
The Journal of Clinical Endocrinology & Metabolism 
21-06-2017
Imatinib discontinuation in chronic myeloid leukaemia patients with undetectable BCR-ABL transcript level: A systematic review and a meta-analysis
Campiotti L, Suter MB, Guasti L, Piazza R, Grandi AM
European Journal of Cancer, Elsevier vol. 77, 48-56.  
30-03-2017
Metastatic pituitary carcinoma in an SDHB mutation positive patient
Tufton N, Roncaroli F, Hadjudemetriou I, Dang MN, Denes J, Guasti L, Thom M, Baldeweg SE
Endocrine Abstracts, Bioscientifica 
16-03-2017
Pituitary Carcinoma in a Patient with an SDHB Mutation.
Tufton N, Roncaroli F, Hadjidemetriou I, Dénes J, Guasti L, Thom M, Baldeweg SE
Endocr Pathol vol. 28 (4), 320-325.  
10-03-2017
Generation of kisspeptin-responsive GnRH neurons from human pluripotent stem cells
Poliandri A, Miller D, Howard S, Nobles M, Ruiz-Babot G, Harmer S, Tinker A, McKay T et al.
Molecular and Cellular Endocrinology, Elsevier vol. 447, 12-22.  
21-02-2017
Pituitary carcinoma in a patient with germline Succinate Dehydrogenase B mutation
Roncaroli F, Tufton N, Hadjidemetriou I, Dang MD, Guasti L, Baldeweg E, Fersht N
118th Meeting of the British-Neuropathological-Society. 
01-02-2017
DISCOVERY OF MUTATIONS IN MULTIPLE GENES CONTROLLING GNRH NEURONAL MIGRATION AND DEVELOPMENT IN PATIENTS WITH SELF-LIMITED DELAYED PUBERTY
Howard SR, Andre V, Guasti L, Cabrera CP, Barnes MR, Cariboni A, Dunkel L
 
01-01-2017
MECHANISMS OF FIBROBLAST GROWTH FACTOR 21 (FGF21) MEDIATED GROWTH HORMONE RESISTANCE IN HUMAN GROWTH PLATE IN CHRONIC CHILDHOOD CONDITIONS
Mistry JN, Ruiz-Babot G, Zaman F, Guasti L
 
01-01-2017

2016

Efficacy and safety of two dosages of canrenone as add‐on therapy in hypertensive patients taking ace‐inhibitors or angiotensin II receptor blockers and hydrochlorothiazide at maximum dosage in a randomized clinical trial: The ESCAPE‐IT trial
Derosa G, Maffioli P, D'Avino M, Sala C, Mugellini A, Vulpis V, Felis S, Guasti L et al.
Cardiovascular Therapeutics, Wiley vol. 35 (1), 47-54.  
13-12-2016
Mutations in SGPL1, encoding sphingosine-1-phosphate lyase, cause a novel form of primary adrenal insufficiency with steroid resistant nephrotic syndrome
Prasad R, Maharaj A, Meimaridou E, van VP, Buonocore F, Barbagelata E, Bergada I, Cassinelli H et al.
Endocrine Abstracts, Bioscientifica 
14-11-2016
Towards reconstruction of epithelialized cartilages from autologous adipose tissue-derived stem cells
GUASTI L
Journal of Tissue Engineering and Regenerative Medicine, Wiley: 12 Months 
01-11-2016
Mutations in SGPL1, encoding sphingosine-1-phosphate lyase, cause a novel form of primary adrenal insufficiency with steroid resistant nephrotic syndrome
Prasad R, Maharaj A, Meimaridou E, VanVeldhoven P, Buonocore F, Bergada I, Barbagelata E, Cassinelli H et al.
 
14-10-2016
LGR4 and EAP1 mutations are implicated in the phenotype of self-limited delayed puberty
Mancini A, Howard SR, Ruiz-Babot G, Cabrera CP, Barnes MR, Guasti L, Dunkel L
 
14-10-2016
The mechanistic role of fibroblast growth factor 21 in growth hormone resistance secondary to chronic childhood conditions
Narendra MJ, Ruiz-Babot G, Guasti L
 
14-10-2016
Generation of human urine-derived steroidogenic cells through lineage conversion: A new technology to study the adrenal gland
Ruiz-Babot G, Hadjidemetriou I, Ajodha SJ, Ghataore L, Taylor D, Guasti L
Endocrine Abstracts, Bioscientifica 
14-10-2016
Loss of Mrap2 is associated with Sim 1 deficiency and increased circulating cholesterol
Novoselova TV, Larder R, Rimmington D, Lelliott C, Wynn E, Gorrigan RJ, Tate P, Guasti L et al.
Journal of Endocrinology, JOE-16-0057-JOE-16-0057.  
22-04-2016
IGSF10 mutations dysregulate gonadotropin‐releasing hormone neuronal migration resulting in delayed puberty
HOWARD SR, Guasti L, Ruiz-Babot G, Mancini A, David A, Storr H, Metherell LA, Sternberg MJE et al.
Embo Molecular Medicine, Wiley vol. 8 (6), 626-42.  
13-04-2016
Role of IGSF10 mutations in self-limited delayed puberty
Howard S, Guasti L, Ruiz-Babot G, Mancini A, David A, Storr H, Metherell L, Sternberg M et al.
 
01-02-2016

2015

Mutations in HS6ST1 cause self-limited delayed puberty (DP) in addition to idiopathic hypogonadotropic hypogonadism (IHH)
Howard S, Poliandre A, Storr HL, Metherell LA, Cabrera C, Warren H, Barnes M, Wehkalampi K et al.
Endocrine Abstracts, Bioscientifica 
04-11-2015
Mutations in IGSF10 cause self-limited delayed puberty
Howard S, Guasti L, Ruiz-Babot G, Mancini A, David A, Storr H, Metherell L, Cabrera C et al.
Endocrine Abstracts, Bioscientifica 
04-11-2015
Lineage conversion of human cells to an adrenocortical phenotype: a new technology to study the adrenal gland
Babot GR, Hadjidemetriou I, Ajodha SJ, Taylor D, Taylor N
Endocrine Abstracts, Bioscientifica 
12-10-2015
Unveiling the complexity of the undifferentiated zone in the human adrenal cortex
Hadjidemetriou I, Ruiz-Babot G, Gomez-Sanchez C
Endocrine Abstracts, Bioscientifica 
12-10-2015
Mutations in HS6ST1 are causal in self-limited delayed puberty as well as idiopathic hypogonadotropic hypogonadism
Howard S, Poliandri A, Storr H, Metherell L, Cabrera C, Barnes M, Warren H, Wehkalampi K et al.
Endocrine Abstracts, Bioscientifica 
12-10-2015
Mutations in IGSF10 cause self-limited delayed puberty, via disturbance of GnRH neuronal migration
Howard S, Guasti L, Ruiz-Babot G, Mancini A, David A, Storr H, Metherell L, Sternberg M et al.
Endocrine Abstracts, Bioscientifica 
12-10-2015
New Directions for the Treatment of Adrenal Insufficiency
Ruiz-Babot G, Hadjidemetriou I
Frontiers in Endocrinology, Frontiers vol. 6 
06-05-2015

2014

Elevated FGF21 leads to attenuated postnatal linear growth in preterm infants through GH resistance in chondrocytes.
Guasti L, Silvennoinen S, Bulstrode NW, Ferretti P
J Clin Endocrinol Metab vol. 99 (11), E2198-E2206.  
01-11-2014
Expression of Sonic hedgehog signalling components in the developing human adrenal cortex
Katugampola H, Halder W, Ganatra R, Dunkel L, Storr H, Guasti L, King P
Endocrine Abstracts, Bioscientifica 
14-10-2014
Association between statin-induced creatine kinase elevation and genetic polymorphisms in SLCO1B1, ABCB1 and ABCG2
Ferrari M, Guasti L, Maresca A, Mirabile M, Grandi AM, Marino F
European Journal of Clinical Pharmacology, Springer Nature vol. 70 (5), 539-547.  
06-03-2014
A novel gene affecting the timing of puberty
Howard S, Guasti L, Storr H, Metherell L, Cariboni A, Barnes M, Cabrera C, Wehkalampi K et al.
Endocrine Abstracts, Bioscientifica 
01-03-2014
Chondrogenic differentiation of adipose tissue-derived stem cells within nanocaged POSS-PCU scaffolds: a new tool for nanomedicine.
Guasti L, Vagaska B, Seifalian AM, Ferretti P
Nanomedicine vol. 10 (2), 279-289.  
01-02-2014

2013

Dlk1 upregulates Gli1 expression in male rat adrenal capsule cells through the activation of β1 integrin and ERK1-2.
Guasti L, Cavlan D, Cogger K, Banu Z, King PJ
Endocrinology, Endocrine Society vol. 154 (12), 4675-4684.  
24-09-2013
Diagnostic accuracy of lung ultrasound for pulmonary embolism: a systematic review and meta‐analysis
Squizzato A, Rancan E, Dentali F, Bonzini M, Guasti L
Journal of Thrombosis and Haemostasis, Elsevier vol. 11 (7), 1269-1278.  
01-07-2013
Familial glucocorticoid deficiency: New genes and mechanisms.
Meimaridou E, Hughes CR, Kowalczyk J, Guasti L, Chapple JP, King PJ
Mol Cell Endocrinol vol. 371 (1-2), 195-200.  
22-05-2013
FGF signalling through Fgfr2 isoform IIIb regulates adrenal cortex development.
Guasti L, Candy Sze WC, McKay T, Grose R, King PJ
Mol Cell Endocrinol vol. 371 (1-2), 182-188.  
22-05-2013
Familial glucocorticoid deficiency: New genes and mechanisms
Meimaridou E, Hughes CR, Kowalczyk J, Guasti L, Chapple JP
Molecular and Cellular Endocrinology vol. 371 (1-2), 195-200.  
22-05-2013
Certoparin for the treatment and prevention of thrombosis: pharmacological profile and results from clinical studies
Donadini MP, Ageno W, Guasti L
Expert Opinion On Drug Metabolism & Toxicology, Taylor & Francis vol. 9 (7), 901-909.  
27-04-2013
Adrenocortical development, maintenance, and disease.
Yates R, Katugampola H, Cogger K, Meimaridou E, Metherell L, Guasti L
Curr Top Dev Biol vol. 106, 239-312.  
01-01-2013
Soluble RAGE Plasma Levels in Patients with Coronary Artery Disease and Peripheral Artery Disease
Falcone C, Bozzini S, D’Angelo A, Capettini AC, Paganini EM, Falcone R
The Scientific World Journal, Wiley vol. 2013 (1) 
01-01-2013

2012

The role of primary cilia in mouse adrenal and zebrafish interrenal development
Cogger K, Guasti L, Ashworth R, Brennan C, Beales P, Marion V, King P
Cilia, Springer Nature vol. 1 (Suppl 1) 
01-11-2012
A homozygous glutathione peroxidase 1 mutation, p. Arg130-Leu133del, in a patient with familial glucocorticoid deficiency
Kowalczyk⁎ J, Guasti L, Lei XG, Clark AJL
 
01-09-2012
Left ventricular remodeling in patients with metabolic syndrome: Influence of gender
Nicolini E, Martegani G, Maresca AM, Marchesi C, Dentali F, Lazzarini A, Speroni S
Nutrition Metabolism and Cardiovascular Diseases, Elsevier vol. 23 (8), 771-775.  
07-07-2012
Mutations in NNT encoding nicotinamide nucleotide transhydrogenase cause familial glucocorticoid deficiency.
Meimaridou E, Kowalczyk J, Guasti L, Hughes CR, Wagner F, Nürnberg P, Banerjee R
Nat Genet vol. 44 (7), 740-742.  
27-05-2012
High plasticity of pediatric adipose-derived stem cells: too much for selective skeletogenic differentiation?
Guasti L, Prasongchean W, Kleftouris G, Mukherjee S
Stem Cells Trans Med vol. 1 (5), 384-395.  
01-05-2012
MCM4 mutation causes adrenal failure, short stature, and natural killer cell deficiency in humans
Hughes CR, Guasti L, Chuang C-H, Schimenti JC, King PJ, Costigan C, Metherell LA
Journal of Clinical Investigation vol. 122 (3), 814-820.  
01-01-2012
Plasma levels of matrix metalloproteinases and their inhibitors in hypertension
Marchesi C, Dentali F, Nicolini E, Maresca AM, Tayebjee MH, Franz M, Venco A
Journal of Hypertension, Wolters Kluwer vol. 30 (1), 3-16.  
01-01-2012

2011

OSA, metabolic syndrome and CPAP: Effect on cardiac remodeling in subjects with abdominal obesity
Grandi AM, Laurita E, Marchesi C, Maresca AM, Solbiati F, Bernasconi A, Marogna M, Guasti L
Respiratory Medicine, Elsevier vol. 106 (1), 145-152.  
06-11-2011
The E3 ubiquitin ligase Mahogunin ubiquitinates the melanocortin 2 receptor.
Cooray SN, Guasti L, Clark AJL
Endocrinology vol. 152 (11), 4224-4231.  
01-11-2011
Localisation of the melanocortin-2-receptor and its accessory proteins in the developing and adult adrenal gland.
Gorrigan RJ, Guasti L, Clark AJ
J Mol Endocrinol vol. 46 (3), 227-232.  
01-06-2011
Localization of Sonic hedgehog secreting and receiving cells in the developing and adult rat adrenal cortex.
Guasti L, Paul A, Laufer E
Mol Cell Endocrinol vol. 336 (1-2), 117-122.  
10-04-2011
Neutrophils and clinical outcomes in patients with acute coronary syndromes and/or cardiac revascularisation
Guasti L, Dentali F, Castiglioni L, Maroni L, Marino F, Squizzato A, Ageno W, Gaudio G et al.
Thrombosis and Haemostasis, Thieme vol. 106 (04), 591-599.  
01-01-2011

2010

Characterization of aryl hydrocarbon receptor interacting protein (AIP) mutations in familial isolated pituitary adenoma families.
Igreja S, Chahal HS, King P, Bolger GB, Srirangalingam U, Guasti L, Gueorguiev M, Guegan K et al.
Hum Mutat vol. 31 (8), 950-960.  
01-08-2010

2009

Statins, fibrates, and venous thromboembolism: a meta-analysis
Squizzato A, Galli M, Romualdi E, Dentali F, Kamphuisen PW, Guasti L
European Heart Journal, Oxford University Press (Oup) vol. 31 (10), 1248-1256.  
22-12-2009
Angiotensin Type 1 Receptor Expression and Interleukin-8 Production in Polymorphonuclear Leukocytes of Patients With Peripheral Arterial Disease
Marino F, Guasti L, Tozzi M, Maio RC, Castiglioni L, Rasini E, Schembri L, Maroni L et al.
Journal of Cardiovascular Pharmacology, Wolters Kluwer vol. 54 (6), 520-525.  
01-12-2009
Minocycline treatment inhibits microglial activation and alters spinal levels of endocannabinoids in a rat model of neuropathic pain.
Guasti L, Richardson D, Jhaveri M, Eldeeb K, Barrett D, Elphick MR, Alexander SPH, Kendall D et al.
Mol Pain vol. 5 
01-07-2009
MRAP and MRAP2 are bidirectional regulators of the melanocortin receptor family.
Chan LF, Webb TR, Chung T-T, Meimaridou E, Cooray SN, Guasti L, Chapple JP, Egertová M et al.
Proc Natl Acad Sci U S A vol. 106 (15), 6146-6151.  
14-04-2009

2008

Lack of relationship between cardiovascular reactivity to mental stress and autonomic modulation of the sinoatrial node in normotensive and hypertensive male subjects
Guasti L, Simoni C, Mainardi L, Crespi C, Cimpanelli MG, Klersy C, Gaudio G, Codari R et al.
International Journal of Psychophysiology, Elsevier vol. 71 (3), 258-263.  
31-10-2008
ERG voltage-gated K+ channels regulate excitability and discharge dynamics of the medial vestibular nucleus neurones
Pessia M, Servettini I, Panichi R, Guasti L, Arcangeli A
Journal of Physiology vol. 586 (20), 4877-4890.  
23-10-2008
Hedgehog signalling in endocrine development and disease
King PJ, Laufer E
J Endocrinol vol. 198 (3), 439-450.  
01-09-2008
Identification of a posttranslational mechanism for the regulation of hERG1 K+ channel expression and hERG1 current density in tumor cells.
Guasti L, Crociani O, Redaelli E, Pillozzi S, Polvani S, Masselli M, Mello T, Wymore RS
Mol Cell Biol vol. 28 (16), 5043-5060.  
01-08-2008

2007

An unusual case presenting with hypertensive crisis
Guasti L, Simoni C, Scamoni C, Sarzi Braga S, Crespi C, Cimpanelli M, Pedretti R, Mainardi LT et al.
Internal and Emergency Medicine, Springer Nature vol. 2 (1) 
01-03-2007
ERG conductance expression modulates the excitability of ventral horn GABAergic interneurons that control rhythmic oscillations in the developing mouse spinal cord
Furlan F, Taccola G, Grandolfo M, Guasti L, Nistri A
Journal of Neuroscience vol. 27 (4), 919-928.  
24-01-2007

2006

Angiotensin-converting Enzyme Inhibitors Influence Left Ventricular Mass and Function Independently of the Antihypertensive Effect
Grandi AM, Laurita E, Solbiati F, Marchesi C, Nicolini E
Journal of Cardiovascular Pharmacology, Wolters Kluwer vol. 48 (5), 207-211.  
01-11-2006
Mixed cranial nerve neuroma revealing itself as baroreflex failure
Guasti L, Simoni C, Scamoni C, Braga SS, Crespi C, Cimpanelli M, Gaudio G, Pedretti R et al.
Autonomic Neuroscience, Elsevier vol. 130 (1-2), 57-60.  
22-06-2006
Species diversity and peptide toxin blocking selectively of ERG subfamily K+ channels in the central nervous system.
Restano R, Korolkova Y, Diochot S, Gurrola G, Guasti L, Possani L, Arcangeli A
Mol Pharm vol. 69 (5), 1673-83.  
01-05-2006
Angiotensin II type 1 receptor expression on human leukocyte subsets: A flow cytometric and RT-PCR study
Rasini E, Cosentino M, Marino F, Legnaro M, Ferrari M, Guasti L
Peptides, Elsevier vol. 134 (2-3), 69-74.  
13-03-2006
Metabolic Syndrome and Morphofunctional Characteristics of the Left Ventricle in Clinically Hypertensive Nondiabetic Subjects
Grandi AM, Maresca AM, Giudici E, Laurita E, Marchesi C, Solbiati F, Nicolini E
American Journal of Hypertension, Oxford University Press (Oup) vol. 19 (2), 199-205.  
01-02-2006
Thyroid hormone and thyrotropin regulate intracellular free calcium concentrations in human polymorphonuclear leukocytes: in vivo and in vitro studies.
Marino F, Guasti L, Cosentino M, Piazza DD, Simoni C, Bianchi V, Piantanida E, Saporiti F et al.
International Journal of Immunopathology and Pharmacology, Sage Publications vol. 19 (1), 149-160.  
01-01-2006

2005

Expression pattern of the ether-a-go-go-related (ERG) family proteins in the adult mouse central nervous system: evidence for coassembly of different subunits.
Guasti L, Cilia E, Crociani O, Polvani S, Becchetti A, Tempia F
J Comp Neurol vol. 491 (2), 157-174.  
17-10-2005
Thyroid hormone regulation of cell migration and oxidative metabolism in polymorphonuclear leukocytes: Clinical evidence in thyroidectomized subjects on thyroxine replacement therapy
Marino F, Guasti L, Cosentino M, De Piazza D, Simoni C, Piantanida E, Klersy C, Bartalena L
Life Sciences, Elsevier vol. 78 (10), 1071-1077.  
09-09-2005
Human ether-a-go-go-related gene 1 channels are physically linked to beta1 integrins and modulate adhesion-dependent signaling.
Cherubini A, Hofmann G, Pillozzi S, Guasti L, Crociani O, Cilia E, Di Stefano P, Balzi M et al.
Mol Biol Cell vol. 16 (6), 2972-2983.  
01-06-2005
Interneurons transiently express the ERG K+ channels during development of mouse spinal networks in vitro
Furlan* F, Guasti* L, Avossa D, Becchetti A, Cilia E, Arcangeli A
Neuroscience vol. 135 (4), 1179-1192.  
01-01-2005

2004

Physical and functional interaction between integrins and hERG potassium channels.
Arcangeli A, Becchetti A, Cherubini A, Crociani O, Defilippi P, Guasti L, Hofmann G, Olivotto M
Biochem Soc Trans vol. 32 (Pt 5), 826-827.  
01-11-2004
Physical and functional interaction between integrins and hERG potassium channels
Arcangeli A, Becchetti A, Cherubini A, Crociani O, Defilippi P, Guasti L, Pillozzi S, Olivotto M
Biochemical Society Transactions vol. 32 (5), 826-827.  
01-11-2004
Natriuretic peptides as markers of preclinical cardiac disease in obesity
Grandi AM, Laurita E, Selva E, Piantanida E, Imperiale D, Guasti L
European Journal of Clinical Investigation, Wiley vol. 34 (5), 342-348.  
01-05-2004
herg1 gene and HERG1 protein are overexpressed in colorectal cancers and regulate cell invasion of tumor cells.
Lastraioli* E, Guasti* L, Crociani O, Polvani S, Hofmann G, Witchel H, Bencini L, Messerini L et al.
Cancer Res vol. 64 (2), 606-611.  
15-01-2004

2003

Cell cycle-dependent expression of HERG1 and HERG1B isoforms in tumor cells.
Crociani O, Guasti L, Balzi M, Becchetti A, Wymore RS
J Biol Chem vol. 278 (5), 2947-2955.  
31-01-2003

2002

Aldosterone Antagonist Improves Diastolic Function in Essential Hypertension
Grandi AM, Imperiale D, Santillo R, Barlocco E, Bertolini A, Guasti L
Hypertension, Wolters Kluwer vol. 40 (5), 647-652.  
01-11-2002
HERG potassium channels are constitutively expressed in primary human acute myeloid leukemias and regulate cell proliferation of normal and leukemic hemopoietic progenitors.
Pillozzi S, Brizzi MF, Balzi M, Crociani O, Cherubini A, Guasti L, Becchetti A, Wanke E et al.
Leukemia vol. 16 (9), 1791-1798.  
01-09-2002
HERG K+ channels and β1 integrins interact through the assembly of a macromolecular complex
Cherubini A, Pillozzi S, Hofmann G, Crociani O, Guasti L, Lastraioli E, Polvani S, Masi A et al.
Annals of The New York Academy of Sciences vol. 973, 559-561.  
01-01-2002

2001

Autonomic function and baroreflex sensitivity during angiotensin-converting enzyme inhibition or angiotensin II AT-1 receptor blockade in essential hypertensive patients
Guasti L, Petrozzino MR, Mainardi LT, Grimoldi P, Zanotta D, Garganico D, Simoni C, Cerutti S
Acta Cardiologica, Peeters Publishers vol. 56 (5), 289-295.  
01-10-2001
HERG K channels activation during β1 integrin-mediated adhesion to fibronectin induces an up regulation of αvβ3 integrin in the preosteoclastic leukemia cell line.
Hofmann G, Bernabei PA, Crociani O, Cherubini A, Guasti L, Lastraioli E, Polvani S, Solazzo V et al.
J Biol Chem, 4923-31.  
01-02-2001

2000

Obesity and left ventricular diastolic function: noninvasive study in normotensives and newly diagnosed never-treated hypertensives
Grandi A, Zanzi P, Piantanida E, Bertolini A
International Journal of Obesity, Springer Nature vol. 24 (8), 954-958.  
01-08-2000
Microalbuminuria in never-treated hypertensives: Lack of relationship to hyperinsulinemia and genetic predisposition to hypertension
Grandi AM, Santillo R, Zanzi P, Broggi R, Imperiale D, Bertolini A, Jessula A, Selva E
American Journal of Hypertension, Oxford University Press (Oup) vol. 13 (4), 353-358.  
01-04-2000

1999

Insulin and Diastolic Dysfunction in Lean and Obese Hypertensives
Grandi AM, Zanzi P, Fachinetti A, Gaudio G, Ceriani L, Guasti L
Hypertension, Wolters Kluwer vol. 34 (6), 1208-1214.  
01-12-1999
Relationship between dental pain perception and 24 hour ambulatory blood pressure
Guasti L, Zanotta D, Petrozzino MR, Diolisi A, Garganico D, Gaudio G, Grandi AM
Journal of Hypertension, Wolters Kluwer vol. 17 (12), 1799-1804.  
01-12-1999
Relationship between a genetic predisposition to hypertension, blood pressure levels and pain sensitivity
Guasti L, Gaudio G, Zanotta D, Grimoldi P, Bertolini A, Grandi AM
Pain, Wolters Kluwer vol. 82 (3), 311-317.  
01-09-1999

Dlk1 is a novel adrenocortical stem/progenitor cell marker that predicts malignancy in adrenocortical carcinoma
Mariniello K, Pittaway JFH, Altieri B, Borges KS, Hadjidemetriou I, Ribeiro C, Ruiz-Babot G, Lim JA et al.
In Biorxiv 
HERG K+ channels activation during beta(1) integrin-mediated adhesion to fibronectin induces an up-regulation of alpha(v)beta(3) integrin in the preosteoclastic leukemia cell line FLG 29.1.
Hoffman G, Bernabei PA, Crociani O, Cherubini A, Guasti L, Pilozzi S, Lastraioli E, Polvani S et al.
Journal of Biological Chemistry, American Society For Biochemistry and Molecular Biology vol. 276, 4923-4931.  
ERG Blockade Enhances Firing and Epinephrine Secretion in Rat Chromaffin Cells: the Missing Link to LQT2-related Sudden Death?
GUASTI L, Gullo F, Ales E, Rosati B
 
SGPL1 mutations cause primary adrenal insufficiency and steroid resistant nephrotic syndrome
METHERELL LA, Guasti L, meimaridou E, maharaj A, storr HL, Hadjidemetriou I
Journal of Clinical Investigation, American Society For Clinical Investigation 
Modeling congenital adrenal hyperplasia and testing interventions for adrenal insufficiency using donor- specific reprogrammed cells.
GUASTI L, Ruiz-Babot G, Balyura M, HADJIDEMETRIOU I, AJODHA S, Taylor D, Ghataore L, Taylor N et al.
Cell Reports, Elsevier 
MRAP deficiency impairs adrenal progenitor cell differentiation and gland zonation
CHAN L
The Faseb Journal 

Grants

solid heart iconGrants of specific relevance to Predictive in vitro Models
bullet iconA 3-way deep investigation of DLK1 as a key modulator and target of ACC to develop innovative target
Leonardo Guasti
£142,904 Nefkens Adrenal Cancer Foundation
01-01-2026 - 31-12-2028
bullet iconA dual origin for pituitary gonadotrophs and its relevance for reproductive function
Karine Rizzoti and Leonardo Guasti
£1,190,752 Medical Research Council
01-12-2025 - 30-06-2029
bullet iconGeneration of functional adrenocortical organoids from mice and humans and their preclinical testing
Leonardo Guasti
£485,578 MRC Medical Research Council
15-04-2024 - 14-04-2027
bullet iconSphingosine-1-phosphate lyase as a novel therapeutic target in Adrenocortical Carcinoma
Rathi Prasad, Leonardo Guasti and Lou Metherell
£334,995 Barts and the London Charity
01-04-2023 - 06-11-2026

bullet iconAdrenal induction of Phe catabolism fuels catecholamine biosynthesis in obstructive sleep apnoea leading to cardiovascular disease
Gabor Czibik, Li Chan and Leonardo Guasti
£74,101 Barts and the London Charity
01-01-2024 - 02-07-2025
bullet iconRecapitulating beta cell development adjacent to the islet vascular niche to advance beta cell replacement therapies for type 1 diabetes
Leonardo Guasti
£794,619 MRC Medical Research Council
01-09-2022 - 31-08-2025
bullet iconCryostat co-funding
Leonardo Guasti and Sasha Howard
£6,700 WHRF William Harvey Research Foundation
01-08-2022 - 31-07-2023
bullet iconThe contribution of capsular and subcapsular progenitor cells in homeostatic adrenal cortex self-renewal and zonal-specific remodelling
Leonardo Guasti, Emanuel Rognoni and Carles Gaston-Massuet
£470,140 BBSRC Biotechnology and Biological Sciences Research Council
01-11-2021 - 31-10-2024
bullet iconAdrenocortical Carcinomas: Searching For Novel Targets For Effective Treatment Modalities (2).
Leonardo Guasti and Peter King
£351,359 Barts and the London Charity
01-09-2018 - 31-01-2023
bullet iconSearching cancer stem cells in adrenocortical carcinoma
Leonardo Guasti
£36,000 Rosetrees Trust
01-02-2018 - 31-01-2021