Dr Adrian Biddle
DHT Lecturer in Animal Replacement Science
CPM Annual Symposium Organiser
Centre for Cell Biology & Cutaneous Research, Blizard Institute
Queen Mary University of London
Queen Mary University of London
Research
cancer stem cells, tumour metastasis, 3D tumour invasion, metastasis on a chip, organ-on-a-chip, machine learning
Interests
Cancer stem cells drive tumour metastasis and therapeutic resistance. Understanding the underlying mechanisms will be crucial if we are to deliver better treatments for cancer, and we model this process using human cancer cell lines in advanced 3D invasion and microfluidic metastasis systems.We also use human tissue archives, which provide a wealth of information on tumour behaviour in the human body, including data on the clinical course of disease. We have developed new ways of obtaining greater information from these tumour specimens, including simultaneous measurement of multiple cancer stem cell markers and a machine learning approach for prognostic prediction.
Publications
2025
A CD24+CD271+ melanoma cancer stem cell possesses hybrid characteristics of its single marker counterparts and promotes invasion and therapeutic resistanceKnowles O, Doldan P, Hillier-Richardson I, Lunetto S, Lunt S, Youssef G, Gammon L, Mackenzie IC et al.
Bmc Biology, Springer Nature vol. 23 (1)
04-08-2025
The nucleosome remodeling and deacetylase-SWItch/sucrose non-fermentable antagonism regulates the coordinated activation of epithelial-to-mesenchymal transition and inflammation in oral cancerStabile R, Tucci FA, Verhagen MP, Embregts C, van den Bosch TPP, Joosten R, De Herdt MJ, van der Steen B et al.
Journal of The National Cancer Institute, Oxford University Press (Oup) vol. 117 (7), 1438-1455.
20-03-2025
2024
Development of an in vitro microfluidic model to study the role of microenvironmental cells in oral cancer metastasisScemama A, Lunetto S, Tailor A, Di Cio S, Dibble M, Gautrot J, Biddle A
F1000research, F1000 Research Ltd vol. 12, 439-439.
15-01-2024
Development of an in vitro microfluidic model to study the role of microenvironmental cells in oral cancer metastasisScemama A, Lunetto S, Tailor A, Di Cio S, Dibble M, Gautrot J, Biddle A
In F1000res
01-01-2024
2023
Disseminating cells in human oral tumours possess an EMT cancer stem cell marker profile that is predictive of metastasis in image-based machine learningYoussef G, Gammon L, Ambler L, Lunetto S, Scemama A, Cottom H, Piper K, Mackenzie IC et al.
Elife, Elife vol. 12
17-11-2023
CD73 controls Myosin II–driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cellsSamain R, Maiques O, Monger J, Lam H, Candido J, George S, Ferrari N, KohIhammer L et al.
Science Advances, American Association For The Advancement of Science (Aaas) vol. 9 (42)
18-10-2023
Development of an in vitro microfluidic model to study the role of microenvironmental cells in oral cancer metastasisScemama A, Tailor A, Di Cio S, Dibble M, Gautrot J, Biddle A
F1000research, F1000 Research Ltd vol. 12, 439-439.
25-04-2023
In vitro cancer models as an approach to identify targetable developmental phenotypes in cancer stem cellsBiddle A
In Vitro Models, Springer Nature vol. 2 (3-4), 83-88.
01-01-2023
2022
Organ-on-a-Chip Technologies Network special issue editorialBiddle A
In Vitro Models, Springer Nature vol. 1 (6), 397-398.
17-11-2022
Cancer stem cell plasticity and its implications in the development of new clinical approaches for oral squamous cell carcinomaMarles H, Biddle A
Biochemical Pharmacology, Elsevier vol. 204
17-08-2022
Highlight: microfluidic devices for cancer metastasis studiesScemama A, Lunetto S, Biddle A
In Vitro Models, Springer Nature vol. 1 (6), 399-403.
27-06-2022
Interconnected high-dimensional landscapes of epithelial–mesenchymal plasticity and stemness in cancerSahoo S, Ashraf B, Duddu AS, Biddle A, Jolly MK
Clinical & Experimental Metastasis, Springer Nature vol. 39 (2), 279-290.
07-01-2022
2020
Portrait of a CAF: The story of cancer-associated fibroblasts in head and neck cancerCustódio M, Biddle A, Tavassoli M
Oral Oncology, Elsevier vol. 110
01-10-2020
2019
585 Identifying cancer stem cells in patient specimens through high-content imaging - biological insights and prognostic potentialYoussef G, Cottoms H, Piper K, Gammon L, Knowles O, Lunt SA, Mackenzie I, Philpott M et al.
01-09-2019
2018
Hybrid epithelial/mesenchymal phenotypes promote metastasis and therapy resistance across carcinomasJolly MK, Somarelli JA, Sheth M, Biddle A, Tripathi SC, Armstrong AJ, Hanash SM, Bapat SA et al.
Pharmacology & Therapeutics, Elsevier vol. 194, 161-184.
28-09-2018
Effects of Cetuximab and Erlotinib on the behaviour of cancer stem cells in head and neck squamous cell carcinomaMACKENZIE IC, Rodrigues MF, Gammon L, RAHMAN M, BIDDLE A, Nunes FD
Oncotarget, Impact Journals
05-02-2018
Identification of a novel cancer stem cell subpopulation in melanoma pathological specimensKnowles O, Doldan P, Hillier-Richardson I, Lunt S, Gammon L, Youssef G, Harwood C, Mackenzie I et al.
01-01-2018
2017
Reprogramming to developmental plasticity in cancer stem cellsO’Brien-Ball C, Biddle A
Developmental Biology, Elsevier vol. 430 (2), 266-274.
31-07-2017
The amoeboid cell in cancerVig NK, Gammon L, Peyric E, Rahman M
01-01-2017
2016
Phenotypic Plasticity Determines Cancer Stem Cell Therapeutic Resistance in Oral Squamous Cell CarcinomaBIDDLE A, Gammon L, Liang X, Costea DE, Mackenzie IC
Ebiomedicine, Elsevier: Creative Commons
15-01-2016
2015
Invasive oral cancer stem cells display resistance to ionising radiationGemenetzidis E, Gammon L, Biddle A, Emich H, Mackenzie IC
Oncotarget, Impact Journals vol. 6 (41), 43964-43977.
02-11-2015
Elevation in 5-FU-induced apoptosis in head and neck cancer stem cells by a combination of CDHP and GSK3β inhibitors.Shigeishi H, Biddle A, Gammon L, Rodini CO, Yamasaki M, Seino S, Sugiyama M, Takechi M et al.
J Oral Pathol Med vol. 44 (3), 201-207.
01-03-2015
Phenotypic plasticity and epithelial‐to‐mesenchymal transition in the behaviour and therapeutic response of oral squamous cell carcinomaVig N, Mackenzie IC, Biddle A
Journal of Oral Pathology and Medicine, Wiley vol. 44 (9), 649-655.
16-01-2015
2014
Implications of the Cancer Stem Cell (CSC) Paradigm for OMFS Patients and SurgeonsVig N, Biddle A, Gammon L, Mackenzie I
British Journal of Oral and Maxillofacial Surgery, Elsevier vol. 52 (8)
01-10-2014
2013
Maintenance of stem cell self-renewal in head and neck cancers requires actions of GSK3β influenced by CD44 and RHAMM.Shigeishi H, Biddle A, Gammon L, Emich H, Rodini CO, Gemenetzidis E, Fazil B, Sugiyama M et al.
Stem Cells vol. 31 (10), 2073-2083.
01-10-2013
CD44 staining of cancer stem-like cells is influenced by down-regulation of CD44 variant isoforms and up-regulation of the standard CD44 isoform in the population of cells that have undergone epithelial-to-mesenchymal transition.Biddle A, Gammon L, Fazil B, Mackenzie IC
Plos One vol. 8 (2)
01-01-2013
Sub-sets of cancer stem cells differ intrinsically in their patterns of oxygen metabolism.Gammon L, Biddle A, Heywood HK, Johannessen AC, Mackenzie IC
Plos One vol. 8 (4)
01-01-2013
Expression of Betapapillomavirus Oncogenes Increases the Number of Keratinocytes with Stem Cell-Like PropertiesHufbauer M, Biddle A, Borgogna C, Gariglio M, Doorbar J, Mackenzie I
Journal of Virology vol. 87 (22), 12158-12165.
01-01-2013
2012
Cancer stem cells and EMT in carcinoma.Biddle A, Mackenzie IC
Cancer Metastasis Rev
03-02-2012
Pre-culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potentialVALORANI MG, Montelatici E, Germani A, Biddle A, D'Alessandro D, Strollo R, Lazzari L, Nye E
Cell Proliferation vol. 45 (3), 225-238.
01-01-2012
2011
Cancer stem cells in squamous cell carcinoma switch between two distinct phenotypes that are preferentially migratory or proliferative.Biddle A, Liang X, Gammon L, Fazil B, Harper LJ, Emich H, Costea DE, Mackenzie IC
Cancer Res vol. 71 (15), 5317-5326.
01-08-2011
Stem cell characteristics of cell sub-populations in cell lines derived from head and neck cancers of Fanconi anemia patients.Gammon L, Biddle A, Fazil B, Harper L, Mackenzie IC
J Oral Pathol Med vol. 40 (2), 143-152.
01-02-2011
2010
Hypoxia increases Sca-1/CD44 co-expression in murine mesenchymal stem cells and enhances their adipogenic differentiation potential.Valorani MG, Germani A, Otto WR, Harper L, Biddle A, Khoo CP, Lin WR, Hawa MI et al.
Cell Tissue Res vol. 341 (1), 111-120.
01-07-2010
Normal and malignant epithelial cells with stem-like properties have an extended G2 cell cycle phase that is associated with apoptotic resistance.Harper LJ, Costea DE, Gammon L, Fazil B, Biddle A, Mackenzie IC
Bmc Cancer vol. 10
28-04-2010
2009
Xenopus oocytes reactivate muscle gene transcription in transplanted somatic nuclei independently of myogenic factorsBiddle A, Simeoni I
Development vol. 136 (16), 2695-2703.
15-08-2009
2005
Bexarotene and Erlotinib for Aerodigestive Tract CancerDragnev KH, Petty WJ, Shah S, Biddle A, Desai NB, Memoli V, Rigas JR, Dmitrovsky E
Journal of Clinical Oncology, American Society of Clinical Oncology (Asco) vol. 23 (34), 8757-8764.
01-12-2005
A Novel Retinoic Acid Receptor β Isoform and Retinoid Resistance in Lung CarcinogenesisPetty WJ, Li N, Biddle A, Bounds R, Nitkin C, Ma Y, Dragnev KH, Freemantle SJ et al.
Journal of The National Cancer Institute, Oxford University Press (Oup) vol. 97 (22), 1645-1651.
16-11-2005
2004
Epidermal Growth Factor Receptor Tyrosine Kinase Inhibition Represses Cyclin D1 in Aerodigestive Tract CancersPetty WJ, Dragnev KH, Memoli VA, Ma Y, Desai NB, Biddle A, Davis TH, Nugent WC et al.
Clinical Cancer Research, American Association For Cancer Research (Aacr) vol. 10 (22), 7547-7554.
15-11-2004
Disseminating cells in human oral tumours acquire an EMT cancer stem cell state that is predictive of metastasisYoussef G, Gammon L, Ambler L, Lunetto S, Scemama A, Cottom H, Piper K, Mackenzie IC et al.
In Biorxiv
A CD24+CD271+ Melanoma Cancer Stem Cell Possesses Hybrid Characteristics of its Single Marker Counterparts and Promotes Invasion and Therapeutic ResistanceKnowles O, Doldan P, Hillier-Richardson I, Lunt S, Youssef G, Gammon L, Mackenzie IC, Philpott MP et al.
In Biorxiv
Hybrid cancer stem cells utilise vascular tracks for collective streaming invasion in a metastasis-on-a-chip deviceIn Biorxiv
A “ tug-of-war” between the NuRD and SWI/SNF chromatin remodelers regulates the coordinated activation of Epithelial-Mesenchymal Transition and inflammation in oral cancerIn Biorxiv
Grants
Grants of specific relevance to Predictive in vitro ModelsThe influence of a complex in vitro tumour microenvironment on cancer stem cells metastasisAdrian Biddle and Ana Caetano
£547,066 National Centre for the Replacement, Refinement and Reduction of Animals in Research
01-05-2025 - 30-04-2028
Using spatial transcriptomics to uncover the molecular mechanisms of tumour progression in oral cancAdrian Biddle
£9,372 Royal College of Surgeons
01-03-2025 - 28-02-2026
The role of Laminin 332 as predictor of occult lymph nodes metastasis in early stage oral cancerMayson Mustafa and Adrian Biddle
£5,500 ODRT Oral and Dental Research Trust, The
04-11-2024 - 03-11-2025
The Role of Peripheral Neuroglia In Oral Spuamous Cell CancerAna Caetano and Adrian Biddle
£317,529 MCSBHT Medical College of St Bartholomew's Hospital Trust
01-10-2024 - 31-12-2027
EPSRC Centre for Doctoral Training in Next Generation Organ-on-a-Chip Technology (COaCT)Hazel Screen, Martin Knight, Thomas Iskratsch, Caroline Roney, Yi Sui, David Lee, Julia Shelton, Zion Tse, John Connelly, Adrian Biddle and Lucy Norling
£7,066,811 Engineering and Physical Sciences Research Council
Grant Summary
01-07-2024 - 30-09-2032
The molecular mechanisms underlying the tumour-determined immune cell infiltrate in oral squamous cell carcinomaAdrian Biddle and Muy-Teck Teh
£298,338 MCSBHT Medical College of St Bartholomew's Hospital Trust
01-09-2021 - 31-01-2026
A Centre of Excellence for Squamous CancerPaul Coulthard, David Kelsell, Catherine Harwood, Adrian Biddle, Jun Wang and Tracy Connelly
£2,675,828 Barts and the London Charity
01-04-2021 - 31-01-2028
PhD Study 2022 – Modelling the tumour-induced changes to the tumour microenvironment (TME) and extracellular matrix (ECM) within a defined minimal 3D in vitro environment.Adrian Biddle
£50,000 Animal Free Research UK
06-06-2022 - 05-06-2025
Identifying and manipulating molecular mechanisms controlling cancer stem cell metastatic potential in a human oral cancer model.Adrian Biddle and Jun Wang
£552,481 MRC Medical Research Council
01-09-2021 - 30-06-2025
A 3Rs approach to tumour metastasis: investigating the role of cancer stem cells in metastasis using an in vitro microfluidic modelAdrian Biddle and Julien Gautrot
£90,000 National Centre for the Replacement, Refinement and Reduction of Animals in Research
01-10-2019 - 30-12-2022
Development of a molecular biomarker test for metastatic cancer stem cells in oral squamous cell carAdrian Biddle
£93,000 Oracle Cancer Trust
02-07-2018 - 01-01-2022
Identification of a novel cancer stem cell sub-population in melanoma pathological specimensAdrian Biddle
£1,940 Animal Free Research UK
04-06-2018 - 03-08-2018