Dr Tom Nightingale

Tom Nightingale
DPhil

Senior Lecturer

The William Harvey Research Institute
Queen Mary University of London

Research

Intracellular Trafficking, Endothelial cells, Haemostasis, Inflammation, Weibel Palade Bodies, Cell Junctions

Cardiovascular Models, Infectious Disease and Inflammation Models

Interests

My interests are in vitro models of the vasculature. We hope to use these to closer investigate the intracellular trafficking events associated with inflammation and blood clotting. Upon endothelial stimulation clotting factors and inflammatory receptors are mobilised from intracellular stores. These are essential for recruiting platelets and leukocytes to sites of injury. We are also interested in understanding how junctions remodel to allow increased permeability and leukocyte transmigration. The vasculature is composed of a number of different cell types and is constantly exposed to flow. This is difficult to model using standard culture models.

Publications

solid heart iconPublications of specific relevance to Predictive in vitro Models

2025

Endothelial β-PIX (ARHGEF7) drives exocytosis through enabling the dynamic reorganisation of the cytoskeleton
El-Mansi S, Deegan KM, Donker L, Godinho S, Nightingale TD
In Cold Spring Harbor Laboratory 
07-05-2025
Probing rare von Willebrand disease–causing mutations in the D4 and C-domains of von Willebrand factor
Mobayen G, El-Mansi S, Chion A, Nightingale TD, McKinnon TAJ
Research and Practice in Thrombosis and Haemostasis, Elsevier Bv vol. 9 (4), 102922-102922.  
01-05-2025

2024

Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane
El-Mansi S, Mitchell TP, Mobayen G, McKinnon TAJ, Miklavc P, Frick M, Nightingale TD
Blood Advances, American Society of Hematology vol. 8 (17), 4714-4726.  
10-09-2024
Senescent endothelial cells promote pathogenic neutrophil trafficking in inflamed tissues
Rolas L, Stein M, Barkaway A, Reglero-Real N, Sciacca E, Yaseen M, Wang H, Vazquez-Martinez L et al.
Embo Reports, Springer Nature vol. 25 (9), 3842-3869.  
25-06-2024

2023

The unconventional Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane
El-Mansi S, Mitchell TP, Miklavc P, Frick M, Nightingale TD
In Cold Spring Harbor Laboratory 
12-08-2023
Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flow
Bosseboeuf E, Chikh A, Chaker AB, Mitchell TP, Vignaraja D, Rajendrakumar R, Khambata RS, Nightingale TD et al.
Science Signaling, American Association For The Advancement of Science (Aaas) vol. 16 (786) 
23-05-2023
Proximity proteomics identifies septins and PAK2 as decisive regulators of actomyosin-mediated expulsion of von Willebrand factor
El-Mansi S, Robinson CL, Kostelnik KB, McCormack JJ, Mitchell TP, Lobato-Márquez D, Rajeeve V, Cutillas P et al.
Blood, American Society of Hematology vol. 141 (8), 930-944.  
23-02-2023

2022

Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescence
Carpintero-Fernández P, Borghesan M, Eleftheriadou O, Pan-Castillo B, Fafián-Labora JA, Mitchell TP, Yuste A, Ogrunc M et al.
Cell Death &Amp; Disease, Springer Science and Business Media Llc vol. 13 (2) 
19-02-2022

2021

The coagulation factor IX (F9) loss of function prevents the cell cycle arrest induced by CDK4/6 inhibitors treatment
Carpintero-Fernández P, Borghesan M, Eleftheriadou O, Fafián-Labora JA, Mitchell TP, Nightingale TD, Mayán MD, O’Loghlen A
In Cold Spring Harbor Laboratory 
30-11-2021
Proximity interactome of LC3B in normal growth conditions
Nollet M, Agrotis A, Michailidis F, Dokal AD, Rajeeve V, Burden J, Nightingale TD, Cutillas P et al.
In Cold Spring Harbor Laboratory 
09-10-2021
Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation
Reglero-Real N, Pérez-Gutiérrez L, Yoshimura A, Rolas L, Garrido-Mesa J, Barkaway A, Pickworth C, Saleeb RS et al.
Immunity, Elsevier Bv vol. 54 (9), 1989-2004.e9.  
01-09-2021
Emerging mechanisms to modulate VWF release from endothelial cells
El-Mansi S, Nightingale TD
The International Journal of Biochemistry &Amp; Cell Biology, Elsevier Bv vol. 131, 105900-105900.  
01-02-2021

2020

Trafficking of JAM-C is regulated by cytoplasmic tail motifs and is essential to endothelial cell migration
Mitchell TP, Kostelnik KB, Barker A, Schultz C, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S et al.
 
01-01-2020

2019

Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration
Kostelnik KB, Barker A, Schultz C, Mitchell TP, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S et al.
Plos Biology, Public Library of Science (Plos) vol. 17 (12) 
02-12-2019
NMDA receptor modulation of glutamate release in activated neutrophils
del Arroyo AG, Hadjihambi A, Sanchez J, Turovsky E, Kasymov V, Cain D, Nightingale TD, Lambden S et al.
Ebiomedicine, Elsevier Bv vol. 47, 457-469.  
01-09-2019
Dynamic trafficking and turnover of Jam-C is essential for endothelial cell migration
Kostelnik KB, Barker A, Schultz C, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S, Cutillas P et al.
In Cold Spring Harbor Laboratory 
02-05-2019

2018

Tuning the endothelial response: differential release of exocytic cargos from Weibel‐Palade bodies
Nightingale TD, McCormack JJ, Grimes W, Robinson C, Lopes da Silva M, White IJ, Vaughan A, Cramer LP et al.
Journal of Thrombosis and Haemostasis, Elsevier Bv vol. 16 (9), 1873-1886.  
01-09-2018
Decrease in Von-Willebrand factor protein in the endothelium of human coronary atherosclerotic plaques: Possible mechanisms and role in thrombosis
Tarvala U, El Matary R, Nightingale T, Poston R
 
01-08-2018
F-actin and septin coordinate to drive the exocytosis of a pro-haemostatic molecule from endothelial cells
Robinson CL, McCormack JJ, White IJ, Cutler DF, Nightingale TD
 
01-01-2018

2017

Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodies
Stevenson NL, White IJ, McCormack JJ, Robinson C, Cutler DF, Nightingale TD
Journal of Cell Science, The Company of Biologists vol. 130 (15), 2591-2605.  
01-08-2017

2016

JAM‐C deficiency primes endothelial cells for a pro‐inflammatory state
Reglero N, Beal R, Cabrera C, Pickworth C, Golding M, Whiteford J, Nightingale T, Imhof B et al.
 
01-04-2016

2013

The secretion of von Willebrand factor from endothelial cells; an increasingly complicated story
Nightingale T, Cutler D
Journal of Thrombosis and Haemostasis, Elsevier Bv vol. 11, 192-201.  
01-06-2013

2012

Actin coats and rings promote regulated exocytosis
Nightingale TD, Cutler DF, Cramer LP
Trends in Cell Biology, Elsevier Bv vol. 22 (6), 329-337.  
01-06-2012

2011

Actomyosin II contractility expels von Willebrand factor from Weibel–Palade bodies during exocytosis
Nightingale TD, White IJ, Doyle EL, Turmaine M, Harrison-Lavoie KJ, Webb KF, Cramer LP, Cutler DF
Journal of Cell Biology, Rockefeller University Press vol. 194 (4), 613-629.  
15-08-2011
Myosin Va Acts in Concert with Rab27a and MyRIP to Regulate Acute Von‐Willebrand Factor Release from Endothelial Cells
Rojo Pulido I, Nightingale TD, Darchen F, Seabra MC, Cutler DF, Gerke V
Traffic, Wiley vol. 12 (10), 1371-1382.  
05-08-2011
Nightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF. Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood. 2009;113(20):5010–5018.
Nightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF
Blood, American Society of Hematology vol. 117 (12), 3476-3476.  
24-03-2011
A role for Rab10 in von Willebrand factor release discovered by an AP‐1 interactor screen in C. elegans
MICHAUX G, DYER CEF, NIGHTINGALE TD, GALLAUD E, NURRISH S, CUTLER DF
Journal of Thrombosis and Haemostasis, Elsevier Bv vol. 9 (2), 392-401.  
01-02-2011

2009

Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells
Nightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF
Blood, American Society of Hematology vol. 113 (20), 5010-5018.  
14-05-2009
A Mechanism of Sialylation Functionally Silences the Hyaluronan Receptor LYVE-1 in Lymphatic Endothelium
Nightingale TD, Frayne MEF, Clasper S, Banerji S, Jackson DG
J Biol Chem vol. 284 (6), 3935-3945.  
06-02-2009

2008

Aftiphilin and γ-Synergin Are Required for Secretagogue Sensitivity of Weibel-Palade Bodies in Endothelial Cells
Lui-Roberts WWY, Ferraro F, Nightingale TD, Cutler DF
Molecular Biology of The Cell, American Society For Cell Biology (Ascb) vol. 19 (12), 5072-5081.  
01-12-2008
Formation and function of Weibel-Palade bodies
Metcalf DJ, Nightingale TD, Zenner HL, Lui-Roberts WW, Cutler DF
Journal of Cell Science, The Company of Biologists vol. 121 (1), 19-27.  
01-01-2008

2005

Functional regulation of the lymphatic hyaluronan receptor (LYVE-1) by terminal sialic acid.
Nightingale TD, Banerji S, Jackson DG
 
01-01-2005

Grants

solid heart iconGrants of specific relevance to Predictive in vitro Models
bullet iconNovel Regulatory Mechanisms for VWF secretion: Towards Therapeutic Control
Tom Nightingale and Mathieu-Benoit Voisin
£286,032 BHF British Heart Foundation
01-02-2023 - 31-01-2026
bullet iconUnderstanding the role of Syndecan-4 in neovascular eye disease
James Whiteford and Tom Nightingale
£323,174 Barts and the London Charity
01-12-2022 - 30-09-2027
bullet iconAdvancing Mesenchymal Stromal Cell-Dressing Therapy for Myocardial Repair
Ken Suzuki, Tom Nightingale, Mauro Perretti, Federica Marelli-Berg, Michael Barnes, Anthony Mathur and Fiona Lewis
£1,263,590 BHF British Heart Foundation
01-09-2020 - 31-05-2026


bullet iconNew approaches to limit angiogenic responses by controlling endothelial junctional remodelling
Tom Nightingale and James Whiteford
£233,773 Barts and the London Charity
01-09-2020 - 31-01-2024
bullet iconControlling the haemostatic response: Novel regulatory mechanisms for Von Willebrand factor secretion from endothelial cells
Tom Nightingale
£232,656 BHF British Heart Foundation
01-02-2020 - 02-11-2023
bullet iconUsing a pathway dynamics approach to identify novel EP4 receptor agonists for the treatment of cardiac inflammation and fibrosis (iCASE matching)
Peter McCormick, Lesley Howell and Tom Nightingale
£107,727 Barts and the London Charity
01-10-2019 - 30-09-2023
bullet iconInvestigation into novelregulatory mechanism for Von Willebrand factor secretion from endothelial cells
Tom Nightingale
£200,752 BHF British Heart Foundation
18-04-2016 - 17-04-2019
bullet iconThe role of Jam-king in endothelial vascular function
Tom Nightingale
£389,244 MRC Medical Research Council
14-09-2015 - 13-10-2018