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Dr Tom Nightingale

Tom NightingaleD Phil

Senior Lecturer

The William Harvey Research Institute
Faculty of Medicine and Dentistry, Queen Mary University of London

www.qmul.ac.uk/whri/people/academic-staff/items/nightingaletom.html

Research

Intracellular Trafficking, Endothelial cells, Haemostasis, Inflammation, Weibel Palade Bodies, Cell Junctions

Cardiovascular Models, Infectious Disease and Inflammation Models

Interests

My interests are in vitro models of the vasculature. We hope to use these to closer investigate the intracellular trafficking events associated with inflammation and blood clotting. Upon endothelial stimulation clotting factors and inflammatory receptors are mobilised from intracellular stores. These are essential for recruiting platelets and leukocytes to sites of injury. We are also interested in understanding how junctions remodel to allow increased permeability and leukocyte transmigration. The vasculature is composed of a number of different cell types and is constantly exposed to flow. This is difficult to model using standard culture models.

Publications

Publications of specific relevance to Predictive in vitro Models

2021

Reglero-Real N, Pérez-Gutiérrez L, Yoshimura A, Rolas L, Garrido-Mesa J, Barkaway A, Pickworth C, Saleeb RS, Gonzalez-Nuñez M, Austin-Williams SN, Cooper D, Vázquez-Martínez L, Fu T, De Rossi G, Golding M, Voisin MB, Boulanger CM, Kubota Y, Muller WA, Tooze SA, Nightingale TD, Collinson L, Perretti M, Aksoy E and Nourshargh S (2021). Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation. Immunity  vol. 54, (9) 1989-2004.e9. 10.1016/j.immuni.2021.07.012
El-Mansi S and Nightingale TD (2021). Emerging mechanisms to modulate VWF release from endothelial cells. International Journal of Biochemistry and Cell Biology  vol. 131, 10.1016/j.biocel.2020.105900

2019

del Arroyo AG, Hadjihambi A, Sanchez J, Turovsky E, Kasymov V, Cain D, Nightingale TD, Lambden S, Grant SGN, Gourine AV and Ackland GL (2019). NMDA receptor modulation of glutamate release in activated neutrophils. Elsevier Bv  Ebiomedicine  10.1016/j.ebiom.2019.08.004
Kostelnik KB, Barker A, Schultz C, Mitchell TP, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S, Cutillas P and Nightingale TD (2019). Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration. Plos Biology  vol. 17, (12) 10.1371/journal.pbio.3000554

2018

NIGHTINGALE TD, McCormack JJ, Grimes W, ROBINSON C, Lopes da Silva M, White IJ, Vaughan A, Cramer LP and Cutler DF (2018). Tuning the endothelial response: differential release of exocytic cargos from Weibel-Palade Bodies. Wiley  Journal of Thrombosis and Haemostasis  10.1111/jth.14218

2017

NIGHTINGALE TD, CUTLER DF, Robinson C, McCormack JJ, White IJ and Stevenson NL (2017). Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodies., Editors: Schiavo G. Company of Biologists  Journal of Cell Science  10.1242/jcs.200840

2013

Nightingale T and Cutler D (2013). The secretion of von Willebrand factor from endothelial cells; an increasingly complicated story. Journal of Thrombosis and Haemostasis  vol. 11, (SUPPL.1) 192-201. 10.1111/jth.12225

2012

Nightingale TD, Cutler DF and Cramer LP (2012). Actin coats and rings promote regulated exocytosis. Trends in Cell Biology  vol. 22, (6) 329-337. 10.1016/j.tcb.2012.03.003

2011

Rojo Pulido I, Nightingale TD, Darchen F, Seabra MC, Cutler DF and Gerke V (2011). Myosin Va acts in concert with Rab27a and MyRIP to regulate acute von-Willebrand factor release from endothelial cells. Traffic  vol. 12, (10) 1371-1382. 10.1111/j.1600-0854.2011.01248.x
Nightingale TD, Pattni K, Hume AN, Seabra MC and Cutler DF (2011). Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. (Blood (2009) 113, 20, (5010-5018)). Blood  vol. 117, (12) 10.1182/blood-2011-01-329136
NIGHTINGALE TD, White IJ, Doyle EL, Turmaine M, Harrison-Lavoie KJ, Webb KF, Cramer LP and Cutler DF (2011). Actomyosin II contractility expels von Willebrand factor from Weibel–Palade bodies during exocytosis. J Cell Biol.  vol. 194, (4) 613-629. 10.1083/jcb.201011119
Michaux G, Dyer CEF, Nightingale TD, Gallaud E, Nurrish S and Cutler DF (2011). A role for Rab10 in von Willebrand factor release discovered by an AP-1 interactor screen in C. elegans. Journal of Thrombosis and Haemostasis  vol. 9, (2) 392-401. 10.1111/j.1538-7836.2010.04138.x

2009

Nightingale TD, Frayne MEF, Clasper S, Banerji S and Jackson DG (2009). A mechanism of sialylation functionally silences the hyaluronan receptor LYVE-1 in lymphatic endothelium. Journal of Biological Chemistry  vol. 284, (6) 3935-3945. 10.1074/jbc.M805105200
NIGHTINGALE TD, Pattni K, Hume AN, Seabra MC and Cutler DF (2009). Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood  vol. 113, (20) 5010-5018. 10.1182/blood-2008-09-181206

2008

Lui-Roberts WWY, Ferraro F, Nightingale TD and Cutler DF (2008). Aftiphilin and γ-synergin are required for secretagogue sensitivity of weibel-palade bodies in endothelial cells. Molecular Biology of The Cell  vol. 19, (12) 5072-5081. 10.1091/mbc.E08-03-0301
Metcalf DJ, Nightingale TD, Zenner HL, Lui-Roberts WW and Cutler DF (2008). Formation and function of Weibel-Palade bodies. Journal of Cell Science  vol. 121, (1) 19-27. 10.1242/jcs.03494

Grants

Grants of specific relevance to Predictive in vitro Models
Advancing mesenchymal stromal cell-dressing therapy for myocardial repair
Nightingale TD
£1,263,556 British Heart Foundation (01-09-2020 - 01-09-2025)
New approaches to limit angiogenic responses by controlling endothelial junctional remodelling
Mitchell T and Nightingale TD
£233,772 Barts and the London Charity (01-12-2020 - 01-12-2023)
Using a pathway dynamics approach to identify novel EP4 receptor agonists for the treatment of cardiac inflammation and fibrosis
Nightingale TD
£107,726 Barts and the London Charity (01-10-2019 - 30-09-2023)
Controlling the haemostatic response: Novel regulatory mechanisms for von willebrand factor secretion from endothelial cells
El-Mansi S and Nightingale TD
£229,656 British Heart Foundation (01-02-2020 - 01-02-2023)
The role of Jam-C trafficking in endothelial vascular function
Kostelnik K and Nightingale TD
£497,748 Medical Research Council (14-09-2015 - 13-10-2022)
The role of CD148 in vascular permeability
Nightingale TD
£85,000 Medical Research Council (01-10-2018 - 30-09-2022)


Incorporating the circadian clock into Organ-on-a-Chip (OOAC) devices
Nightingale TD
£19,669 Organ on a chip (01-08-2020 - 01-11-2020)
Understanding the vascular permeability response in cardiovascular disease using a novel biochemical and bioinformatic approach
Mitchell T and Nightingale TD
£106,000 British Heart Foundation (01-10-2017 - 30-09-2020)
An investigation into novel regulatory mechanisms for Von Willebrand factor secretion from endothelial cells
Robinson C and Nightingale TD
£200,752 British Heart Foundation (18-04-2016 - 17-04-2019)