Dr Pradeepa Madapura

Pradeepa Madapura

Senior Lecturer

Blizard Institute
Faculty of Medicine and Dentistry
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Research

Neurodevelopmental disorders, brain organoids, In vitro brain development, neuroepigenetics, functional genomics

Cardiovascular Models, Neuronal Models

Interests

We use human stem cell-derived cerebral organoid models to investigate the mechanism through which mutations in epigenetic regulators contribute to neurodevelopmental and cardiovascular disorders.

Publications

Publications of specific relevance to Predictive in vitro Models

2024

Jayakumar S, Patel M, Boulet F, Aziz H, Brooke GN, Tummala H and Pradeepa MM (2024). PSIP1/LEDGF reduces R-loops at transcription sites to maintain genome integrity. Springer Nature  Nature Communications  vol. 15, (1) 10.1038/s41467-023-44544-w

2023

Pal D, Patel M, Boulet F, Sundarraj J, Grant OA, Branco MR, Basu S, Santos SDM, Zabet NR, Scaffidi P and Pradeepa MM (2023). H4K16ac activates the transcription of transposable elements and contributes to their cis-regulatory function. Springer Nature  Nature Structural & Molecular Biology  vol. 30, (7) 935-947. 10.1038/s41594-023-01016-5
De Vas MG, Boulet F, Joshi SS, Garstang MG, Khan TN, Atla G, Parry D, Moore D, Cebola I, Zhang S, Cui W, Lampe AK, Lam WW, Consortium GER, Ferrer J, Pradeepa MM and Atanur SS (2023). Regulatory de novo mutations underlying intellectual disability. Life Science Alliance, Llc  Life Science Alliance  vol. 6, (5) 10.26508/lsa.202201843

2022

Rodriguez-Algarra F, Seaborne RAE, Danson AF, Yildizoglu S, Yoshikawa H, Law PP, Ahmad Z, Maudsley VA, Brew A, Holmes N, Ochôa M, Hodgkinson A, Marzi SJ, Pradeepa MM, Loose M, Holland ML and Rakyan VK (2022). Genetic variation at mouse and human ribosomal DNA influences associated epigenetic states. Springer Nature  Genome Biology  vol. 23, (1) 10.1186/s13059-022-02617-x

2021

Sundarraj J, Taylor GCA, von Kriegsheim A and Pradeepa MM (2021). H3K36me3 and PSIP1/LEDGF associate with several DNA repair proteins, suggesting their role in efficient DNA repair at actively transcribing loci. Faculty of 1000  Wellcome Open Research  vol. 2, 10.12688/wellcomeopenres.11589.4
Olley G, Pradeepa MM, Grimes GR, Piquet S, Polo SE, FitzPatrick DR, Bickmore WA and Boumendil C (2021). Cornelia de Lange syndrome-associated mutations cause a DNA damage signalling and repair defect. Springer Nature  Nature Communications  vol. 12, (1) 10.1038/s41467-021-23500-6

2019

Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park S-M, Bickmore WA, Pradeepa MM and FitzPatrick DR (2019). Publisher Correction: BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome. Springer Nature  Nature Genetics  vol. 51, (7) 1192-1192. 10.1038/s41588-019-0448-1

2018

Garstang MG and Pradeepa MM (2018). An Enhancer-Derived RNA Muscles In to Regulate Myogenin In trans. Mol Cell  vol. 71, (1) 3-5. 10.1016/j.molcel.2018.06.024
Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park S-M, Bickmore WA, Pradeepa MM, FitzPatrick DR and Deciphering Developmental Disorders Study (2018). Publisher Correction: BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome. Springer Nature  Nature Genetics  vol. 50, (5) 767-767. 10.1038/s41588-018-0069-0
Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park S-M, Deciphering Developmental Disorders Study , Bickmore WA, Pradeepa MM and FitzPatrick DR (2018). BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome. Springer Nature  Nature Genetics  vol. 50, (3) 329-332. 10.1038/s41588-018-0042-y

2017

Marin R, Cortez D, Lamanna F, Pradeepa MM, Leushkin E, Julien P, Liechti A, Halbert J, Brüning T, Mössinger K, Trefzer T, Conrad C, Kerver HN, Wade J, Tschopp P and Kaessmann H (2017). Convergent origination of a Drosophila-like dosage compensation mechanism in a reptile lineage. Cold Spring Harbor Laboratory  Genome Research  vol. 27, (12) 1974-1987. 10.1101/gr.223727.117
Pradeepa MM, Taylor GCA and von Kriegsheim A (2017). ­­­Proteomic analysis of H3K36me3 and PSIP1/p75 (LEDGF) complexes reveal their wider role in DNA repair. Faculty of 1000  Wellcome Open Research  vol. 2, 10.12688/wellcomeopenres.11589.1
Pradeepa MM, McKenna F, Taylor GCA, Bengani H, Grimes GR, Wood AJ, Bhatia S and Bickmore WA (2017). Psip1/p52 regulates posterior Hoxa genes through activation of lncRNA Hottip. Public Library of Science (Plos)  Plos Genetics  vol. 13, (4) 10.1371/journal.pgen.1006677
Pradeepa MM (2017). Causal role of histone acetylations in enhancer function. Transcription  vol. 8, (1) 40-47. 10.1080/21541264.2016.1253529

2016

Marulasiddappa P, Pradeepa MM, Kumar Y, Grimes G, Illingworth R and Bickmore W (2016). Native chromatin immunoprecipitation of histone marks to Identify enhancer status. Springer Nature  Protocol Exchange  10.1038/protex.2016.033
Pradeepa MM, Grimes GR, Kumar Y, Olley G, Taylor GCA, Schneider R and Bickmore WA (2016). Histone H3 globular domain acetylation identifies a new class of enhancers. Nature Genetics  vol. 48, (6) 681-686. 10.1038/ng.3550
Robertson KA, Hsieh WY, Forster T, Blanc M, Lu H, Crick PJ, Yutuc E, Watterson S, Martin K, Griffiths SJ, Enright AJ, Yamamoto M, Pradeepa MM, Lennox KA, Behlke MA, Talbot S, Haas J, Dölken L, Griffiths WJ, Wang Y, Angulo A and Ghazal P (2016). An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway. Public Library of Science (Plos)  Plos Biology  vol. 14, (3) 10.1371/journal.pbio.1002364

2015

Illingworth RS, Moffat M, Mann AR, Read D, Hunter CJ, Pradeepa MM, Adams IR and Bickmore WA (2015). The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development. Cold Spring Harbor Laboratory  Genes & Development  vol. 29, (18) 1897-1902. 10.1101/gad.268151.115
Gupta N, Madapura MP, Bhat UA and Rao MRS (2015). Mapping of Post-translational Modifications of Transition Proteins, TP1 and TP2, and Identification of Protein Arginine Methyltransferase 4 and Lysine Methyltransferase 7 as Methyltransferase for TP2*. Elsevier  Journal of Biological Chemistry  vol. 290, (19) 12101-12122. 10.1074/jbc.m114.620443

2014

Pradeepa MM, Grimes GR, Taylor GCA, Sutherland HG and Bickmore WA (2014). Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Oxford University Press (Oup)  Nucleic Acids Research  vol. 42, (14) 9021-9032. 10.1093/nar/gku647

2013

Taylor GCA, Eskeland R, Hekimoglu-Balkan B, Pradeepa MM and Bickmore WA (2013). H4K16 acetylation marks active genes and enhancers of embryonic stem cells, but does not alter chromatin compaction. Cold Spring Harbor Laboratory  Genome Research  vol. 23, (12) 2053-2065. 10.1101/gr.155028.113

2012

Pradeepa MM, Sutherland HG, Ule J, Grimes GR and Bickmore WA (2012). Psip1/Ledgf p52 Binds Methylated Histone H3K36 and Splicing Factors and Contributes to the Regulation of Alternative Splicing. Public Library of Science (Plos)  Plos Genetics  vol. 8, (5) 10.1371/journal.pgen.1002717

2009

Pradeepa MM, Nikhil G, Kishore AH, Bharath GN, Kundu TK and Rao MRS (2009). Acetylation of Transition Protein 2 (TP2) by KAT3B (p300) Alters Its DNA Condensation Property and Interaction with Putative Histone Chaperone NPM3*. Elsevier  Journal of Biological Chemistry  vol. 284, (43) 29956-29967. 10.1074/jbc.m109.052043
Kolthur-Seetharam U, Pradeepa MM, Gupta N, Narayanaswamy R and Rao MRS (2009). Spatiotemporal Organization of AT- and GC-rich DNA and Their Association With Transition Proteins TP1 and TP2 in Rat Condensing Spermatids. Sage Publications  Journal of Histochemistry & Cytochemistry  vol. 57, (10) 951-962. 10.1369/jhc.2009.953414

2008

Pradeepa MM, Manjunatha S, Sathish V, Agrawal S and Rao MRS (2008). Involvement of Importin-4 in the Transport of Transition Protein 2 into the Spermatid Nucleus. Taylor & Francis  Molecular and Cellular Biology  vol. 28, (13) 4331-4341. 10.1128/mcb.00519-07

2007

Pradeepa MM and Rao MRS (2007). Chromatin remodeling during mammalian spermatogenesis: role of testis specific histone variants and transition proteins. Society of Reproduction and Fertility  vol. 63, 1-10.
Dhar S, Pradeepa MM and Rao MRS (2007). Emerging concepts of chromatin remodeling in modulating genome functions. Proceedings of The National Academy of Sciences India Section B-Biological Sciences  vol. 77, 61-71.