Dr Matthew Caley
Senior Lecturer in Cell Biology
Research
Skin, Lipids, rare diseases, screening, Extracellular Matrix, Laminin 332
Interests
My research focusses on the skin basement membrane and specifically laminin 332 a three-chained glycoprotein which is copiously secreted by epithelial cells. We have demonstrated an important role for laminin 332 in skin ageing, squamous cell carcinoma invasion, cholesterol transport and in skin immune regulation. To study these diverse functions we have generated co-culture and 3D skin models including models of aged skin that have allowed us to test gene knockdowns and drug treatments. We have also developed and validated a high throughput screen to identify compounds altering cholesterol transport within the skin.Publications
Publications of specific relevance to Predictive in vitro Models2023
Garcha P, Jones E, Shelley J and Caley M (2023). P32 Restoring the ageing skin barrier in vitro. Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) e26-e27. 10.1093/bjd/ljad174.053Summan SK, Mayes A, Gunn D, Gautrot J, Caley M and O’Toole E (2023). P16 How does skin ageing-related basement membrane collagen loss affect the nucleus? Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) e20-e20. 10.1093/bjd/ljad174.037Parzymies P, Jones EM, Mayes A, Messenger D, O’Toole E and Caley M (2023). O16 The novel roles of laminin α3 in epidermal barrier function and metabolism. Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) e11-e11. 10.1093/bjd/ljad174.016Giusto E, Enjalbert F, Rognoni E, Caley M and O’Toole E (2023). O13 JAK inhibitors to restore skin barrier function in ARCI. Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) e9-e10. 10.1093/bjd/ljad174.013Jones E, Marsh S, O'Shaughnessy R, Aumailley M, McGrath J, O’Toole E and Caley M (2023). O14 Junctional epidermolysis bullosa: repairing the epidermal lipid barrier. Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) e10-e10. 10.1093/bjd/ljad174.014Lohana P, Suryaprawira A, Woods EL, Dally J, Gait-Carr E, Alaidaroos NYA, Heard CM, Lee KY, Ruge F, Farrier JN, Enoch S, Caley MP, Peake MA, Davies LC, Giles PJ, Thomas DW, Stephens P and Moseley R (2023). Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. Antioxidants vol. 12, (7) 10.3390/antiox12071374Hindle S, Taggart E, Blowes L, Pennington D, Singh B, Caley M and Connelly J (2023). 1035 Understanding dynamic immune responses within a 3D microfluidic model of human skin. Elsevier Journal of Investigative Dermatology vol. 143, (5) 10.1016/j.jid.2023.03.1046Tawfik SS, Thomas B, Jones E, Caley M, O'Shaughnessy R and O'Toole E (2023). 1157 Ozone as an aggravating factor in atopic eczema. Elsevier Journal of Investigative Dermatology vol. 143, (5) 10.1016/j.jid.2023.03.1170Jones E, Marsh S, Aumailley M, McGrath JA, O'Toole E, Rognoni E and Caley M (2023). 753 Repairing the epidermal skin barrier in an in vivo model of junctional epidermolysis bullosa. Elsevier Journal of Investigative Dermatology vol. 143, (5) 10.1016/j.jid.2023.03.762Summan SK, Mayes A, Gunn DA, Gautrot J, Caley M and O'Toole E (2023). 741 The effect of skin ageing-related basement membrane collagen loss on the nucleus. Elsevier Journal of Investigative Dermatology vol. 143, (5) s127-s127. 10.1016/j.jid.2023.03.750Parzymies PJ, Jones E, Mayes A, Messenger D, O'Toole E and Caley M (2023). 763 Novel roles of laminin α3 in epidermal homeostasis. Elsevier Journal of Investigative Dermatology vol. 143, (5) 10.1016/j.jid.2023.03.772Smith CJ, Williams JL, Hall C, Casas J, Caley MP, O'Toole EA, Prasad R and Metherell LA (2023). Ichthyosis linked to sphingosine 1-phosphate lyase insufficiency is due to aberrant sphingolipid and calcium regulation. Journal of Lipid Research vol. 64, (4) 10.1016/J.JLR.2023.100351Frost JM, Amante SM, Okae H, Jones EM, Ashley B, Lewis RM, Cleal JK, Caley MP, Arima T, Maffucci T and Branco MR (2023). Regulation of human trophoblast gene expression by endogenous retroviruses. Nature Structural and Molecular Biology vol. 30, (4) 527-538. 10.1038/s41594-023-00960-6McGeoghan F, Camera E, Maiellaro M, Menon M, Huang M, Dewan P, Ziaj S, Caley MP, Donaldson M, Enright AJ and O’Toole EA (2023). RNA sequencing and lipidomics uncovers novel pathomechanisms in recessive X-linked ichthyosis. Frontiers in Molecular Biosciences vol. 10, 10.3389/fmolb.2023.11768022022
Dina DO, Caley MP, Raj N, O’Toole EA and Connelly J (2022). 216 Understanding the Mechanisms of Epidermal Barrier Damage and Repair Using 3D Skin Equivalent Models. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.227Jones E, Marsh S, O’Shaughnessy R, Aumailley M, McGrath JA, O’Toole EA and Caley MP (2022). 191 Junctional Epidermolysis Bullosa:Repairing The Epidermal Skin Barrier. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.202Tawfik SS, Thomas B, Jones E, Caley MP, O’Shaughnessy R and O’Toole EA (2022). 144 Carbonylated proteins and Ozone in atopic eczema. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.154Parzymies P, Jones E, Caley MP, O’Toole EA, Messenger D and Mayes A (2022). 184 The role of the Laminin 332 in cholesterol transport in dry and Aged skin. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.195Smith CJ, Williams JL, Hall C, Caley MP, O’Toole EA, Prasad R and Metherell LA (2022). 181 Sphingosine 1-phosphate lyase insufficiency induced ichthyosis is due to sphingolipid imbalance, increased differentiation, and aberrant intercellular junctions. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.192Smith CJ, Parkinson EK, Yang J, Pratten J, O'Toole EA, Caley MP and Braun KM (2022). Investigating wound healing characteristics of gingival and skin keratinocytes in organotypic cultures. Journal of Dentistry vol. 125, 10.1016/j.jdent.2022.1042512021
Smith CJ, Williams JL, Caley MP, O’Toole EA, Prasad R and Metherell LA (2021). 099 Ichthyosis associated with sphingosine 1-phosphate lyase deficiency is due to aberrant calcium and sphingolipid regulation. Journal of Investigative Dermatology vol. 141, (10) s165-s165. 10.1016/j.jid.2021.08.102Caley MP, Martins VL, Moore K, Lashari M, Nissinen L, Kähäri V-M, Alexander S, Jones E, Harwood CA, Jones J, Donaldson M, Marshall JF and O'Toole EA (2021). Loss of the laminin subunit alpha-3 induces cell invasion and macrophage infiltration in cutaneous squamous cell carcinoma. Br J Dermatol vol. 184, (5) 923-934. 10.1111/bjd.19471Langton AK, Ayer J, Griffiths TW, Rashdan E, Naidoo K, Caley MP, Birch-Machin MA, O'Toole EA, Watson REB and Griffiths CEM (2021). Distinctive clinical and histological characteristics of atrophic and hypertrophic facial photoageing. Journal of The European Academy of Dermatology and Venereology vol. 35, (3) 762-768. 10.1111/jdv.170632020
Enjalbert F, Dewan P, Caley MP, Jones EM, Morse MA, Kelsell DP, Enright AJ and O'Toole EA (2020). 3D model of harlequin ichthyosis reveals inflammatory therapeutic targets. American Society For Clinical Investigation Journal of Clinical Investigation 10.1172/JCI1329872019
Hassan S, Purdie KJ, Wang J, Harwood CA, Proby CM, Pourreyron C, Mladkova N, Nagano A, Dhayade S, Athineos D, Caley M, Mannella V, Blyth K, Inman GJ and Leigh IM (2019). A unique panel of patient-derived cutaneous squamous cell carcinoma cell lines provides a preclinical pathway for therapeutic testing. International Journal of Molecular Sciences vol. 20, (14) 10.3390/ijms201434282018
Caley M, Wall IB, Peake M, Kipling D, Giles P, Thomas DW and Stephens P (2018). Development and characterisation of a human chronic skin wound cell line—towards an alternative for animal experimentation. International Journal of Molecular Sciences vol. 19, (4) 10.3390/ijms190410012016
Caley MP, King H, Shah N, Wang K, Rodriguez-Teja M, Gronau JH, Waxman J and Sturge J (2016). Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces. Clinical and Experimental Metastasis vol. 33, (2) 151-165. 10.1007/s10585-015-9765-7Branco MR, King M, Perez-Garcia V, Bogutz AB, Caley M, Fineberg E, Lefebvre L, Cook SJ, Dean W, Hemberger M and Reik W (2016). Maternal DNA Methylation Regulates Early Trophoblast Development. Developmental Cell vol. 36, (2) 152-163. 10.1016/j.devcel.2015.12.0272015
Hill DS, Robinson NDP, Caley MP, Chen M, O'Toole EA, Armstrong JL, Przyborski S and Lovat PE (2015). A novel fully humanized 3D skin equivalent to model early melanoma invasion. Molecular Cancer Therapeutics vol. 14, (11) 2665-2673. 10.1158/1535-7163.MCT-15-0394Kopecki Z, Yang GN, Jackson JE, Melville EL, Calley MP, Murrell DF, Darby IA, O'Toole EA, Samuel MS and Cowin AJ (2015). Cytoskeletal protein Flightless I inhibits apoptosis, enhances tumor cell invasion and promotes cutaneous squamous cell carcinoma progression. Impact Journals Oncotarget vol. 6, (34) 36426-36440. 10.18632/oncotarget.5536Martins VL, Caley MP, Moore K, Szentpetery Z, Marsh ST, Murrell DF, Kim MH, Avari M, McGrath JA, Cerio R, Kivisaari A, Kähäri VM, Hodivala-Dilke K, Brennan CH, Chen M, Marshall JF and O'Toole EA (2015). Suppression of TGFβ and Angiogenesis by Type VII Collagen in Cutaneous SCC. J Natl Cancer Inst vol. 108, (1) 10.1093/jnci/djv293Caley MP, Martins VLC and O'Toole EA (2015). Metalloproteinases and Wound Healing. Adv Wound Care (New Rochelle) vol. 4, (4) 225-234. 10.1089/wound.2014.0581Rodriguez-Teja M, Gronau JH, Breit C, Zhang YZ, Minamidate A, Caley MP, McCarthy A, Cox TR, Erler JT, Gaughan L, Darby S, Robson C, Mauri F, Waxman J and Sturge J (2015). AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival. Journal of Pathology vol. 235, (4) 581-592. 10.1002/path.44852014
Cichoń MA, Szentpetery Z, Caley MP, Papadakis ES, Mackenzie IC, Brennan CH and O'Toole EA (2014). The receptor tyrosine kinase Axl regulates cell-cell adhesion and stemness in cutaneous squamous cell carcinoma. Oncogene vol. 33, (32) 4185-4192. 10.1038/onc.2013.388Peake MA, Caley M, Giles PJ, Wall I, Enoch S, Davies LC, Kipling D, Thomas DW and Stephens P (2014). Identification of a transcriptional signature for the wound healing continuum. Wound Repair Regen vol. 22, (3) 399-405. 10.1111/wrr.121702013
Pellegrino L, Stebbing J, Braga VM, Frampton AE, Jacob J, Buluwela L, Jiao LR, Periyasamy M, Madsen CD, Caley MP, Ottaviani S, Roca-Alonso L, El-Bahrawy M, Coombes RC, Krell J and Castellano L (2013). miR-23b regulates cytoskeletal remodeling, motility and metastasis by directly targeting multiple transcripts. Nucleic Acids Res vol. 41, (10) 5400-5412. 10.1093/nar/gkt245Martins VL, Caley M and O'Toole EA (2013). Matrix metalloproteinases and epidermal wound repair. Cell Tissue Res vol. 351, (2) 255-268. 10.1007/s00441-012-1410-zPalmieri C, Caley MP, Purshouse K, Fonseca A-V, Rodriguez-Teja M, Kogianni G, Woodley L, Odendaal J, Elliott K, Waxman J and Sturge J (2013). Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer. Br J Cancer vol. 108, (1) 163-169. 10.1038/bjc.2012.540Stephens P, Caley M and Peake M (2013). Alternatives for animal wound model systems. Methods Mol Biol vol. 1037, 177-201. 10.1007/978-1-62703-505-7_10Stephens P, Caley M and Peake M (2013). Alternatives for animal wound model systems. Methods in Molecular Biology vol. 1037, 177-201. 10.1007/978-1-62703-505-7_102012
Ramasamy K, Khatun H, Macpherson L, Caley MP, Sturge J, Mufti GJ, Schey SA and Calle Y (2012). Fluorescence-based experimental model to evaluate the concomitant effect of drugs on the tumour microenvironment and cancer cells. Br J Haematol vol. 157, (5) 564-579. 10.1111/j.1365-2141.2012.09103.xCaley MP, Kogianni G, Adamarek A, Gronau JH, Rodriguez-Teja M, Fonseca A-V, Mauri F, Sandison A, Rhim JS, Pchejetski D, Palmieri C, Cobb JP, Waxman J and Sturge J (2012). TGFβ1-Endo180-dependent collagen deposition is dysregulated at the tumour-stromal interface in bone metastasis. J Pathol vol. 226, (5) 775-783. 10.1002/path.39582011
Sturge J, Caley MP and Waxman J (2011). Bone metastasis in prostate cancer: emerging therapeutic strategies. Nat Rev Clin Oncol vol. 8, (6) 357-368. 10.1038/nrclinonc.2011.67Sturge J, Caley MP and Waxmanin J (2011). Authors' reply: Expanding horizons in metastatic prostate cancer treatment. Nature Reviews Clinical Oncology vol. 8, (10) 10.1038/nrclinonc.2011.67-c2Sturge J, Caley MP and Waxman J (2011). Bone metastasis in prostate cancer: emerging therapeutic strategies (vol 8, pg 357, 2011). Nature Reviews Clinical Oncology vol. 8, (10) 568-568. 10.1038/nrclinonc.2011.1272008
Mantripragada KK, Caley M, Stephens P, Jones CJ, Kluwe L, Guha A, Mautner V and Upadhyaya M (2008). Telomerase activity is a biomarker for high grade malignant peripheral nerve sheath tumors in neurofibromatosis type 1 individuals. Genes Chromosomes Cancer vol. 47, (3) 238-246. 10.1002/gcc.20525Mantripragada KK, Caley M, Stephens P, Jones CJ, Kluwe L, Guha A, Mautner V and Upadhyaya M (2008). Telomerase activity is a biomarker for high grade malignant peripheral nerve sheath tumors in neurofibromatosis type I individuals. Genes Chromosomes & Cancer vol. 47, (3) 238-246. 10.1002/gcc.205252007
Caley M, Wall I, Kipling D, Thomas DW and Stephens P (2007). Development and characterisation of a human cell-based chronic wound bioassay. Wound Repair and Regeneration vol. 15, (6) A133-A133.