Dr Jianmin Chen

Jianmin Chen

Versus Arthritis Career Development Fellow

William Harvey Research Institute
Queen Mary University of London

Research

Interests

I am interested to study the switching of phenotypes and alterations in phagocytosis activity of macrophages upon pharmaceutical treatments in vitro. This assay can be conducted with biogel-elicited macrophages or bone marrow-derived macrophages. Phenotypes and phagocytosis activity of macrophages can be determined by flow cytometry. I have also developed techniques to look at mitochondrial mass and potential from isolated primary cardiomyocytes, immune cells, cardiac fibroblasts and endothelial cells using flow cytometry.

Publications

Publications of specific relevance to Predictive in vitro Models

2023

Chen J, Oggero S, Cecconello C, Dalli J, Hayat H, Hjiej Andaloussi A, Sanni S, Jonassen TE and Perretti M (2023). The Annexin-A1 mimetic RTP-026 promotes acute cardioprotection through modulation of immune cell activation. Elsevier  Pharmacological Research  vol. 198, 10.1016/j.phrs.2023.107005
Chen J, Austin-Williams S, O'Riordan CE, Claria-Ribas P, Sugimoto MA, Norling LV, Thiemermann C and Perretti M (2023). Formyl Peptide Receptor Type 2 Deficiency in Myeloid Cells Amplifies Sepsis-Induced Cardiac Dysfunction. Karger Publishers  Journal of Innate Immunity  vol. 15, (1) 548-561. 10.1159/000530284

2022

Costa VV, Sugimoto MA, Hubner J, Bonilha CS, Queiroz-Junior CM, Gonçalves-Pereira MH, Chen J, Gobbetti T, Rodrigues GOL, Bambirra JL, Passos IB, Lopes CEM, Moreira TP, Bonjour K, Melo RC, Oliveira MA, Andrade MVM, Sousa LP, Souza DG, da Costa Santiago H, Perretti M and Teixeira MM (2022). Targeting the Annexin A1-FPR2/ALX pathway for host-directed therapy in dengue disease. Elife  Elife  vol. 11, 10.7554/elife.73853
Hamers A, Primus CP, Whitear C, Kumar NA, Masucci M, Moreira SAM, Rathod K, Chen J, Bubb K, Colas R, Khambata RS, Dalli J and Ahluwalia A (2022). 20‐hydroxyeicosatetraenoic acid (20‐HETE) is a pivotal endogenous ligand for TRPV1‐mediated neurogenic inflammation in the skin. Wiley  British Journal of Pharmacology  vol. 179, (7) 1450-1469. 10.1111/bph.15726

2021

Gee LC, Massimo G, Lau C, Primus C, Fernandes D, Chen J, Rathod KS, Hamers AJP, Filomena F, Nuredini G, Ibrahim AS, Khambata RS, Gupta AK, Moon JC, Kapil V and Ahluwalia A (2021). Inorganic nitrate attenuates cardiac dysfunction: roles for xanthine oxidoreductase and nitric oxide. Wiley  British Journal of Pharmacology  vol. 179, (20) 4757-4777. 10.1111/bph.15636
Chen J, Norling LV, Mesa JG, Silva MDP, Burton SE, Reutelingsperger C, Perretti M and Cooper D (2021). Annexin A1 attenuates cardiac diastolic dysfunction in mice with inflammatory arthritis. Proceedings of The National Academy of Sciences  Proceedings of The National Academy of Sciences of The United States of America  vol. 118, (38) 10.1073/pnas.2020385118
de Gaetano M, Tighe C, Gahan K, Zanetti A, Chen J, Newson J, Cacace A, Marai M, Gaffney A, Brennan E, Kantharidis P, Cooper ME, Leroy X, Perretti M, Gilroy D, Godson C and Guiry PJ (2021). Asymmetric Synthesis and Biological Screening of Quinoxaline-Containing Synthetic Lipoxin A4 Mimetics (QNX-sLXms). American Chemical Society (Acs)  Journal of Medicinal Chemistry  vol. 64, (13) 9193-9216. 10.1021/acs.jmedchem.1c00403

2020

Chen J, Purvis GSD, Collotta D, Zoubi SA, Sugimoto MA, Cacace A, Martin L, Colas RA, Collino M, Dalli J and Thiemermann C (2020). RvE1 Attenuates Polymicrobial Sepsis-Induced Cardiac Dysfunction and Enhances Bacterial Clearance. Frontiers Media  Frontiers in Immunology  vol. 11, 2080-2080. 10.3389/fimmu.2020.02080
Aziz Q, Chen J, Moyes AJ, Li Y, Anderson NA, Ang R, Aksentijevic D, Sebastian S, Hobbs AJ, Thiemermann C and Tinker A (2020). Vascular KATP channels protect from cardiac dysfunction and preserve cardiac metabolism during endotoxemia. Springer Science and Business Media Llc  Journal of Molecular Medicine  10.1007/s00109-020-01946-3
Kieswich JE, Chen J, Alliouachene S, Caton PW, McCafferty K, Thiemermann C and Yaqoob MM (2020). Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome. Bio-Protocol, Llc  Bio-Protocol  vol. 10, (18) 10.21769/bioprotoc.3751

2019

Yun Y, Chen J, Liu R, Chen W, Liu C, Wang R, Hou Z, Yu Z, Sun Y, IJzerman AP, Heitman LH, Yin X and Guo D (2019). Long residence time adenosine A1 receptor agonists produce sustained wash-resistant antilipolytic effect in rat adipocytes. Elsevier  Biochemical Pharmacology  vol. 164, 45-52. 10.1016/j.bcp.2019.03.032

2018

Zoubi SA, Chen J, Murphy C, Martin L, Chiazza F, Collotta D, Yaqoob MM, Collino M and Thiemermann C (2018). Linagliptin Attenuates the Cardiac Dysfunction Associated With Experimental Sepsis in Mice With Pre-existing Type 2 Diabetes by Inhibiting NF-κB. Frontiers  Frontiers in Immunology  vol. 9, 10.3389/fimmu.2018.02996
Kieswich JE, Chen J, Alliouachene S, Caton PW, McCafferty K, Thiemermann C and Yaqoob MM (2018). A novel model of reno-cardiac syndrome in the C57BL/ 6 mouse strain. Springer Nature  Bmc Nephrology  vol. 19, (1) 10.1186/s12882-018-1155-3
de Gaetano M, Butler E, Gahan K, Zanetti A, Marai M, Chen J, Cacace A, Hams E, Maingot C, McLoughlin A, Brennan E, Leroy X, Loscher CE, Fallon P, Perretti M, Godson C and Guiry PJ (2018). Asymmetric synthesis and biological evaluation of imidazole- and oxazole-containing synthetic lipoxin A4 mimetics (sLXms). Elsevier  European Journal of Medicinal Chemistry  vol. 162, 80-108. 10.1016/j.ejmech.2018.10.049
Chen J, Hamers AJP, Finsterbusch M, Massimo G, Zafar M, Corder R, Colas RA, Dalli J, Thiemermann C and Ahluwalia A (2018). Endogenously generated arachidonate-derived ligands for TRPV1 induce cardiac protection in sepsis. Faseb J  fj201701303-fj201701303. 10.1096/fj.201701303R
Khan AI, Kapoor A, Chen J, Martin L, Rogazzo M, Mercier T, Decosterd L, Collino M and Thiemermann C (2018). The Antimalarial Drug Artesunate Attenuates Cardiac Injury in A Rodent Model of Myocardial Infarction. Shock  vol. 49, (6) 675-681. 10.1097/SHK.0000000000000963

2017

Purvis GSD, Chiazza F, Chen J, Loiola RA, Martin L, Fountoulakis N, Gnudi L, Collino M, Thiemermann C and Solito E (2017). Annexin A1 attenuates microvascular complications through restoration of Akt signalling in a murine model of type 1 diabetes. Springer Verlag  Diabetologia  10.1007/s00125-017-4469-y
Johnson FL, Patel NSA, Purvis GSD, Chiazza F, Chen J, Sordi R, Hache G, Merezhko VV, Collino M, Yaqoob MM and Thiemermann C (2017). Inhibition of IκB Kinase at 24 Hours After Acute Kidney Injury Improves Recovery of Renal Function and Attenuates Fibrosis. J Am Heart Assoc  vol. 6, (7) 10.1161/JAHA.116.005092
Martin L, Gombert A, Chen J, Liebens J, Verleger J, Kalder J, Marx G, Jacobs M, Thiemermann C and Schuerholz T (2017). The β-d-Endoglucuronidase Heparanase Is a Danger Molecule That Drives Systemic Inflammation and Correlates with Clinical Course after Open and Endovascular Thoracoabdominal Aortic Aneurysm Repair: Lessons Learnt from Mice and Men. Frontiers Media  Front Immunol  vol. 8, 681-681. 10.3389/fimmu.2017.00681

2016

Chen J and Thiemermann C (2016). Selenium and Niacin for Sepsis Therapy. Wolters Kluwer  Critical Care Medicine  vol. 44, (6) 1256-1257. 10.1097/ccm.0000000000001493
Chen J, Kieswich J, Chiazza F, Moyes A, Gobbetti T, Purvis G, Salvatori D, Patel N, Perretti M, Hobbs A, Collino M, Yaqoob M and THIEMERMANN C (2016). IkB Kinase Inhibitor Attenuates Sepsis-Induced Cardiac Dysfunction in CKD. American Society of Nephrology  Journal of The American Society of Nephrology  10.1681/ASN.2015060670
Zhang W, Zhao X, Xiao Y, Chen J, Han P, Zhang J, Fu H and Kang YJ (2016). The association of depressed angiogenic factors with reduced capillary density in the Rhesus monkey model of myocardial ischemia. Oxford University Press (Oup)  Metallomics  vol. 8, (7) 654-662. 10.1039/c5mt00332f

2014

Gobbetti T, Coldewey SM, Chen J, McArthur S, le Faouder P, Cenac N, Flower RJ, Thiemermann C and Perretti M (2014). Nonredundant protective properties of FPR2/ALX in polymicrobial murine sepsis. Proceedings of The National Academy of Sciences  Proceedings of The National Academy of Sciences of The United States of America  vol. 111, (52) 18685-18690. 10.1073/pnas.1410938111
Chen J, Chiazza F, Collino M, Patel NSA, Coldewey SM and Thiemermann C (2014). Gender Dimorphism of the Cardiac Dysfunction in Murine Sepsis: Signalling Mechanisms and Age-Dependency. Public Library of Science (Plos)  Plos One  vol. 9, (6) 10.1371/journal.pone.0100631

2013

Sun X, Cai J, Fan X, Han P, Xie Y, Chen J, Xiao Y and Kang YJ (2013). Decreases in Electrocardiographic R-Wave Amplitude and QT Interval Predict Myocardial Ischemic Infarction in Rhesus Monkeys with Left Anterior Descending Artery Ligation. Public Library of Science (Plos)  Plos One  vol. 8, (8) 10.1371/journal.pone.0071876

2012

Xie Y, Chen J, Han P, Yang P, Hou J and Kang YJ (2012). Immunohistochemical detection of differentially localized up-regulation of lysyl oxidase and down-regulation of matrix metalloproteinase-1 in rhesus monkey model of chronic myocardial infarction. Frontiers  Experimental Biology and Medicine  vol. 237, (7) 853-859. 10.1258/ebm.2012.012070

Grants

Grants of specific relevance to Predictive in vitro Models
Resolution Pharmacology: targeting metabolism to mitigate the cardiomyopathy of inflammatory arthritis
Chen J
£115,778 British Heart Foundation (09-10-2023 - 09-10-2026)
Targeting heart failure with preserved ejection fraction in settings of arthritis
Chen J
£121,068 Heart Research UK (03-04-2023 - 03-04-2026)
Investigating the cardiac microvasculature in a model of diastolic dysfunction
Chen J
£115,788 British Heart Foundation (20-09-2022 - 20-09-2025)
A novel “two-hit” model of HFpEF induced by arthritis and metabolic syndrome”
Chen J
£9,969 British Pharmacological Society (15-05-2023 - 15-11-2024)

Modelling cardiomyopathy in inflammatory arthritis to define mechanisms and guide therapeutic strategies
Chen J
£469,692 Versus Arthritis UK (18-08-2022 - 17-08-2023)
Cardiomyopathy in RA
Perretti M, Norling L, Cooper D and Chen J
£230,000 Bristol-Myers Squibb (01-06-2021 - 30-11-2022)
Summary
Characterisation of cardiac dysfunction in a mouse model of rheumatoid arthritis
Cooper D, Marelli-Berg F and Chen J
£78,928 Barts Charity (18-11-2019 - 17-05-2021)