Prof Steffi Krause
Professor of Electroanalytical Systems
School of Engineering and Materials Science
Queen Mary University of London
Queen Mary University of London
Research
electrochemistry, photoelectrochemical imaging, functional cell imaging, biosensors
Interests
Our group has developed a photoelectrochemical imaging system for functional imaging of cellular processes in real time and with subcellular resolution. Our measurement technology has been shown to be sensitive to the surface charges and the permeability of cell membranes of cells in culture and is also capable of monitoring the concentrations of ions released by cells.Publications
Publications of specific relevance to Predictive in vitro Models2022
Jacques R, Zhou B, Marhuenda E, Gorecki J, Das A, Iskratsch T and Krause S (2022). Photoelectrochemical imaging of single cardiomyocytes and monitoring of their action potentials through contact force manipulation of organoids. Elsevier Biosensors and Bioelectronics 10.1016/j.bios.2022.115024Zhou B, Jiang Y, Guo Q, Das A, Jorge Sobrido A, Hing K, Zayats A and Krause S (2022). Photoelectrochemical Detection of Calcium Ions Based on Hematite Nanorod Sensors. American Chemical Society Acs Applied Nano Materials 10.1021/acsanm.2c03978Meng Y, Chen F, Wu C, Krause S, Wang J and Zhang DW (2022). Light-Addressable Electrochemical Sensors toward Spatially Resolved Biosensing and Imaging Applications. American Chemical Society Acs Sensors 10.1021/acssensors.2c009402021
Wan K, Liu Y, Santagiuliana G, Barandun G, Taroni Junior P, Güder F, Bastiaansen CWM, Baxendale M, Fenwick O, Papageorgiou DG, Krause S, Zhang H and Bilotti E (2021). Self-powered ultrasensitive and highly stretchable temperature–strain sensing composite yarns. Materials Horizons 10.1039/d1mh00908gZhou B, Das A, Zhong M, Guo Q, Zhang DW, Hing KA, Sobrido AJ, Titirici MM and Krause S (2021). Photoelectrochemical imaging system with high spatiotemporal resolution for visualizing dynamic cellular responses. Biosensors and Bioelectronics vol. 180, 10.1016/j.bios.2021.1131212020
Tu Y, Kyle C, Luo H, Zhang DW, Das A, Briscoe J, Dunn S, Titirici MM and Krause S (2020). Ammonia Gas Sensor Response of a Vertical Zinc Oxide Nanorod-Gold Junction Diode at Room Temperature. Acs Sensors vol. 5, (11) 3568-3575. 10.1021/acssensors.0c017692019
Zhou B, Das A, Kappers M, Oliver R, Humphreys C and Krause S (2019). InGaN as a substrate for AC photoelectrochemical imaging. Mdpi Sensors 10.3390/s19204386Wang J, Yang Z, Chen W, Du L, Jiao B, Krause S, Wang P, Wei Q, Zhang D-W and Wu C (2019). Modulated light-activated electrochemistry at silicon functionalized with metal-organic frameworks towards addressable DNA chips. Elsevier Biosensors and Bioelectronics vol. 146, 111750-111750. 10.1016/j.bios.2019.111750Wan K, Taroni PJ, Liu Z, Liu Y, Tu Y, Santagiuliana G, Hsia I, Zhang H, Fenwick O, Krause S, Baxendale M, Schroeder BC and Bilotti E (2019). Flexible and Stretchable Self‐Powered Multi‐Sensors Based on the N‐Type Thermoelectric Response of Polyurethane/Na
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Composites. Wiley Advanced Electronic Materials 1900582-1900582. 10.1002/aelm.201900582Ahmad N, Colak B, Gibbs MJ, Zhang D-W, Gautrot JE, Watkinson M, Becer CR and Krause S (2019). Peptide Cross-Linked Poly(2-oxazoline) as a Sensor Material for the Detection of Proteases with a Quartz Crystal Microbalance. Biomacromolecules vol. 20, (7) 2506-2514. 10.1021/acs.biomac.9b00245Wu F, Zhou B, Wang J, Zhong M, Das A, Watkinson M, Hing K, Zhang D-W and Krause S (2019). Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges. Anal Chem 10.1021/acs.analchem.9b00304Ahmad N, Colak B, Zhang DW, Gibbs MJ, Watkinson M, Remzi Becer C, Gautrot JE and Krause S (2019). Peptide cross-linked poly (Ethylene glycol) hydrogel films as biosensor coatings for the detection of collagenase. Sensors (Switzerland) vol. 19, (7) 10.3390/s190716772018
Zhang D, Papaioannou N, Titirici M-M and Krause S (2018). Photoelectrochemical Imaging Using Carbon Dots (CDs) Derived from Chitosan. Conference Proceedings vol. 2, (13) 10.3390/proceedings2130778Ahmad N, Colak B, Gibbs MJ, Zhang D-W, Becer CR, Watkinson M, Gautrot JE and Krause S (2018). Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films. 10.3390/proceedings2130961TU Y, AHMAD N, BRISCOE J, ZHANG D and KRAUSE S (2018). Light-Addressable Potentiometric Sensors using ZnO Nanorods as the Sensor Substrate for Bioanalytical Applications. American Chemical Society Analytical Chemistry 10.1021/acs.analchem.8b02244Wang J, Du L, Krause S, Wu C and Wang P (2018). Surface modification and construction of LAPS towards biosensing applications. Sensors and Actuators, B: Chemical vol. 265, 161-173. 10.1016/j.snb.2018.02.190ZHANG D, PAPAIOANNOU N, David NM, LUO H, GAO H, Tanase LC, Degousee T, Samori P, SAPELKIN A, FENWICK O, TITIRICI M and KRAUSE SK (2018). Photoelectrochemical response of carbon dots (CDs) derived from chitosan and their use in electrochemical imaging. Rsc Materials Horizons 10.1039/c7mh00784aTU Y, CHEN S, LI X, GORBACIOVA J, GILLIN WP, KRAUSE S and BRISCOE J (2018). Control of oxygen vacancies in ZnO nanorods by annealing and their influence on ZnO/PEDOT:PSS diode behaviour. Royal Society of Chemistry Journal of Materials Chemistry C 10.1039/C7TC04284AYoshinobu T, Krause S, Miyamoto K-I, Werner CF, Poghossian A, Wagner T and Schöning MJ (2018). (Bio-)chemical Sensing and Imaging by LAPS and SPIM. Label-Free Biosensing 10.1007/5346_2017_22Schöning MJ, Wagner T, Poghossian A, Miyamoto KI, Werner CF, Krause S and Yoshinobu T (2018). Light-addressable potentiometric sensors for (bio-)chemical sensing and imaging. Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry 10.1016/B978-0-12-409547-2.13483-32017
Zhang D, Wu F and KRAUSE SK (2017). LAPS and SPIM imaging using ITO coated glass as the substrate material. American Chemical Society Analytical Chemistry 10.1021/acs.analchem.7b01898Wu F, Campos I, Zhang D and KRAUSE SK (2017). Biological imaging using light-addressable potentiometric sensors and scanning photo-induced impedance microscopy. Royal Society, The Proceedings of The Royal Society a: Mathematical, Physical and Engineering Sciences 10.1098/rspa.2017.0130Zhang D and Krause S (2017). Image Detection of Yeast Saccharomyces Cerevisiae By Light-Addressable Potentiometric Sensors (LAPS). Ecs Meeting Abstracts vol. MA2017-01, (38) 1770-1770. 10.1149/ma2017-01/38/1770Wu F, Watkinson M and Krause S (2017). Electrochemical Imaging of Copper Contamination on Self-Assembled Organic Monolayer Modified Silicon Surfaces Following a ‘Click’ Reaction with Light-Addressable Potentiometric Sensors and Scanning Photo-Induced Impedance Microscopy. Ecs Meeting Abstracts vol. MA2017-01, (38) 1769-1769. 10.1149/ma2017-01/38/1769Wu F, Zhang DW, Wang J, Watkinson M and Krause S (2017). Copper Contamination of Self-Assembled Organic Monolayer Modified Silicon Surfaces Following a “Click” Reaction Characterized with LAPS and SPIM. Langmuir vol. 33, (13) 3170-3177. 10.1021/acs.langmuir.6b03831Krause S (2017). High-resolution impedance and potential imaging using light-addressable potentiometric sensors for bio-imaging applications. 10.11470/jsapmeeting.2017.1.0_2222016
Zhang DW, Wu F, Wang J, Watkinson M and KRAUSE SK (2016). Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS). Electrochemistry Communications vol. 72, 41-45. 10.1016/j.elecom.2016.08.017Wang J, Campos I, Wu F, Zhu J, Sukhorukov GB, Palma M, Watkinson M and Krause S (2016). The effect of gold nanoparticles on the impedance of microcapsules visualized by scanning photo-induced impedance microscopy. Electrochimica Acta vol. 208, 39-46. 10.1016/j.electacta.2016.05.0172015
Wang J, Wu F, Watkinson M, Zhu J and Krause S (2015). click Patterning of Self-Assembled Monolayers on Hydrogen-Terminated Silicon Surfaces and Their Characterization Using Light-Addressable Potentiometric Sensors. Langmuir vol. 31, (35) 9646-9654. 10.1021/acs.langmuir.5b02069Gibbs MJ, Biela A and Krause S (2015). α-Amylase sensor based on the degradation of oligosaccharide hydrogel films monitored with a quartz crystal sensor. Biosens Bioelectron vol. 67, 540-545. 10.1016/j.bios.2014.09.036Zhang H, Yilmaz P, Ansari JO, Khan FF, Binions R, Krause S and Dunn S (2015). Incorporation of Ag nanowires in CuWO4 for improved visible light-induced photoanode performance. Journal of Materials Chemistry A vol. 3, (18) 9638-9644. 10.1039/c4ta07213hBiela A, Watkinson M, Meier UC, Baker D, Giovannoni G, Becer CR and Krause S (2015). Disposable MMP-9 sensor based on the degradation of peptide cross-linked hydrogel films using electrochemical impedance spectroscopy. Biosensors and Bioelectronics vol. 68, 660-667. 10.1016/j.bios.2015.01.0602014
Wang J, Zhou Y, Watkinson M, Gautrot J and Krause S (2014). High-sensitivity light-addressable potentiometric sensors using silicon on sapphire functionalized with self-assembled organic monolayers. Elsevier Sensors and Actuators B: Chemical vol. 209, 230-236. 10.1016/j.snb.2014.11.0712013
Dzaraini K, Norizan A, Krause S, Hunter C and Milanesi L (2013). Degradation behaviour of thin polymer films of poly (amide ester) hydrogel using quartz crystal microbalance. Advanced Materials Research vol. 812, 38-45. 10.4028/www.scientific.net/AMR.812.382012
Zhang DW, Zhang FT, Cui YR, Deng QP, Krause S, Zhou YL and Zhang XX (2012). A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy. Talanta vol. 92, 65-71. 10.1016/j.talanta.2012.01.0492011
Zheng X, Cook JP, Watkinson M, Yang S, Douglas I, Rawlinson A and Krause S (2011). Generic protease detection technology for monitoring periodontal disease. Faraday Discussions vol. 149, 37-47. 10.1039/c005364c2010
Chen L, Zhou Y, Jiang S, Kunze J, Schmuki P and Krause S (2010). High resolution LAPS and SPIM. Electrochemistry Communications vol. 12, (6) 758-760. 10.1016/j.elecom.2010.03.0262009
Kamarun D, Zheng XW, Milanesi L, Hunter CA and Krause S (2009). A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase. Electrochimica Acta vol. 54, (22) 4985-4990. 10.1016/j.electacta.2009.03.067Stair JL, Watkinson M and Krause S (2009). Sensor materials for the detection of proteases. Biosensors and Bioelectronics vol. 24, (7) 2113-2118. 10.1016/j.bios.2008.11.002Sweeney SJ and Krause S (2009). Selected Papers from the Second International Conference on Optical, Optoelectronic and Photonic Materials and Applications, 2007. J Mater Sci-Mater El vol. 20, 1-2. 10.1007/s10854-008-9740-zSweeney SJ and Krause S (2009). Editorial: Selected papers from the second international conference on optical, optoelectronic and photonic materials and applications, 2007. Journal of Materials Science: Materials in Electronics vol. 20, (SUPPL. 1) 10.1007/s10854-008-9740-z2008
Chen L, Zhou Y, Krause S, Munoz AG, Kunze J and Schmuki P (2008). Repair of thin thermally grown silicon dioxide by anodic oxidation. Electrochimica Acta vol. 53, (8) 3395-3402. 10.1016/j.electacta.2007.11.0652007
Zhou Y, Jiang S, Krause S and Chazalviel J-N (2007). Biosensor arrays based on the degradation of thin polymer films interrogated by scanning photoinduced impedance microscopy. Anal Chem vol. 79, (23) 8974-8978. 10.1021/ac071437tZhou Y, Chen L, Krause S and Chazalviel J-N (2007). Scanning photoinduced impedance microscopy using amorphous silicon photodiode structures. Anal Chem vol. 79, (16) 6208-6214. 10.1021/ac070797d2006
Krause S, Moritz W, Talabani H, Xu M, Sabot A and Ensell G (2006). Scanning Photo-Induced Impedance Microscopy - Resolution studies and polymer characterization. Electrochimica Acta vol. 51, (8-9) 1423-1430. 10.1016/j.electacta.2005.02.119KRAUSE SK, Fernandez-Sanchez C and McNeil CJ (2006). Sensors based on thin film degradation. Encyclopedia of Sensors American Scientific Publishers 2004
Moritz W, Yoshinobu T, Finger F, Krause S, Martin-Fernandez M and Schoning MJ (2004). High resolution LAPS using amorphous silicon as the semiconductor material. Sensors and Actuators B-Chemical vol. 103, (1-2) 436-441. 10.1016/j.snb.2004.04.0732003
KRAUSE SK (2003). Impedance methods. Encyclopedia of Electrochemistry, Volume 3, Instrumentation and Electroanalytical Chemistry Wiley-Vch Verlag 10.1002/9783527610426.bard0302062002
Sabot A and Krause S (2002). Simultaneous quartz crystal microbalance impedance and electrochemical impedance measurements. Investigation into the degradation of thin polymer films. Anal Chem vol. 74, (14) 3304-3311. 10.1021/ac0200724Krause S, Talabani H, Xu M, Moritz W and Griffiths J (2002). Scanning photo-induced impedance microscopy - an impedance based imaging technique. Electrochimica Acta vol. 47, (13-14) 2143-2148. 10.1016/S0013-4686(02)00088-92001
Sumner C, Krause S, Sabot A, Turner K and McNeil CJ (2001). Biosensor based on enzyme-catalysed degradation of thin polymer films. Biosensors & Bioelectronics vol. 16, (9-12) 709-714. 10.1016/s0956-5663(01)00210-xMoritz W, Krause S, Roth U, Klimm D and Lippitz A (2001). Re-activation of an all solid state oxygen sensor. Anal Chim Acta vol. 437, (2) 183-190. 10.1016/S0003-2670(01)00993-X2000
Sumner C, Sabot A, Turner K and Krause S (2000). A transducer based on enzyme-induced degradation of thin polymer films monitored by surface plasmon resonance. Anal Chem vol. 72, (21) 5225-5232. 10.1021/ac000411yMoritz W, Gerhardt I, Roden D, Xu M and Krause S (2000). Photocurrent measurements for laterally resolved interface characterization. Fresenius Journal of Analytical Chemistry vol. 367, (4) 329-333. 10.1007/s0021600004091999
Ho WO, Krause S, McNeil CJ, Pritchard JA, Armstrong RD, Athey D and Rawson K (1999). Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation. Anal Chem vol. 71, (10) 1940-1946. 10.1021/ac981367d1998
Moritz W, Krause S, Bartholomäus L, Gabusjan T, Vasiliev AA, Godovski DY and Malyshev VV (1998). Silicon-Based Sensor for Flourine Gas. Acs Symposium Series vol. 690, 119-129. 10.1021/bk-1998-0690.ch0101995
McNeil CJ, Athey D, Ball M, Ho WO, Krause S, Armstrong RD, Des Wright J and Rawson K (1995). Electrochemical Sensors Based on Impedance Measurement of Enzyme-Catalyzed Polymer Dissolution: Theory and Applications. Analytical Chemistry vol. 67, (21) 3928-3935. 10.1021/ac00117a018Moritz W, Krause S, Vasiliev AA, Godovski DY and Malyshev VV (1995). Monitoring of HF and F2 using a field-effect sensor. Sensors and Actuators B Chemical vol. 24, (1-3) 194-196. 10.1016/0925-4005(95)85041-41994
Krause S, Moritz W and Grohmann I (1994). Improved long-term stability for an LaF3 based oxygen sensor. Sensors and Actuators B Chemical vol. 18, (1-3) 148-154. 10.1016/0925-4005(94)87074-81992
Krause S, Moritz W and Grohmann I (1992). A low-temperature oxygen sensor based on the Si/LaF3/Pt capacitive structure. Sensors and Actuators B Chemical vol. 9, (3) 191-196. 10.1016/0925-4005(92)80215-j1991
Bobrov PV, Tarantov YA, Krause S and Moritz W (1991). Chemical sensitivity of an ISFET with Ta2O5 membrane in strong acid and alkaline solutions. Sensors and Actuators B Chemical vol. 3, (1) 75-81. 10.1016/0925-4005(91)85010-gGrants
Grants of specific relevance to Predictive in vitro Models
3D Photoelectrochemical imaging in porous light-addressable structuresKrause SK, Zhou B and Iskratsch T
£202,249 Engineering and Physical Sciences Research Council (01-02-2021 - 31-10-2022)
Combined LAPS and SICM for multimodal live cell imagingWang W, Krause SK and Das A
£571,838 Engineering and Physical Sciences Research Council (01-09-2018 - 31-08-2022)
Light-addressable potentiometric sensors (LAPS) for zinc imaging with high spatiotemporal resolution for elucidating the role of zinc in age related macular degenerationKrause SK and Das A
£146,591 Horizon 2020 (01-07-2017 - 30-06-2019)
Towards in-vivo electrochemical imaging with flexible, light-addressable electrodesKrause SK and Fenwick OJ
£15,000 Royal Society (15-03-2018 - 14-03-2019)
Super-resolution Light-Addressable Potentiometric Sensors (LAPS)Krause SK and Zhang D
£147,661 Biotechnology and Biological Sciences Research Council (20-07-2017 - 19-07-2018)