Dr Ryan O'Shaughnessy
Senior Lecturer in Cell Biology
FMD/Blizard/Centre for Cell Biology and Cutaneous Research
Queen Mary University of London
Queen Mary University of London
Research
Epidermal biology, Epidermal barrier function, Ichthyosis, Squamous Cell Carcinoma, Wound healing, Scarring
Interests
I have a interest in using in vitro models to model disease states and also healthy skin to determine disease mechanisms and also mechanisms important to skin homeostasis. Current relevant expertise is in organotypic, skin equivalent culture, using shRNA knockdown to model human disease.Publications
Publications of specific relevance to Predictive in vitro Models2023
O’Shaughnessy R, Common J and Gutowska-Owsiak D (2023). Editorial: Novel aspects of the immunological and structural barrier of the epidermis. Frontiers Frontiers in Molecular Biosciences vol. 10, 10.3389/fmolb.2023.1324920O’Shaughnessy RFL (2023). Why are squamous cancers so aggressive in recessive dystrophic epidermolysis bullosa? Can we find new therapeutic approaches? Oxford University Press (Oup) British Journal of Dermatology vol. 190, (1) 7-8. 10.1093/bjd/ljad392Jones E, Marsh S, O'Shaughnessy R, Aumailley M, McGrath J, O’Toole E and Caley M (2023). O14 Junctional epidermolysis bullosa: repairing the epidermal lipid barrier. Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) e10-e10. 10.1093/bjd/ljad174.014Tachie-Menson T, Azizah A, Hughes A and O'Shaughnessy R (2023). P27 Uncovering the roles of lysosomal protease cathepsin H in atopic dermatitis. Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) e24-e25. 10.1093/bjd/ljad174.048Hughes A, Barbosa E, Cernova J, Thomas B, O’Toole E and O'Shaughnessy R (2023). P102 Severe atopic eczema is associated with reduced acne vulgaris in Bangladeshi children and young adults: a retrospective analysis from a cross-sectional study. Oxford University Press (Oup) British Journal of Dermatology vol. 188, (Supplement_4) 10.1093/bjd/ljad113.130Tachie-Menson T, Azizah A, Hughes A and O'Shaughnessy R (2023). 770 AKT1-dependent protease control of skin barrier function and inflammation in atopic dermatitis. Elsevier Journal of Investigative Dermatology vol. 143, (5) 10.1016/j.jid.2023.03.779Tawfik SS, Thomas B, Jones E, Caley M, O'Shaughnessy R and O'Toole E (2023). 1157 Ozone as an aggravating factor in atopic eczema. Elsevier Journal of Investigative Dermatology vol. 143, (5) 10.1016/j.jid.2023.03.1170Baruah K, Koh L, Tham K, O'Shaughnessy R, O'Toole E and Common JE (2023). 786 IL-4 stimulates IL-1R2 upregulation in atopic dermatitis keratinocytes. Elsevier Journal of Investigative Dermatology vol. 143, (5) 10.1016/j.jid.2023.03.795Tagoe H, Hassan S, Bliss E, Youssef G, Heywood W, Mills K, Harper JI and O’Shaughnessy RFL (2023). Chronic activation of Toll-like receptor 2 induces an ichthyotic skin phenotype. Oxford University Press (Oup) British Journal of Dermatology vol. 189, (1) 91-102. 10.1093/bjd/ljad0952022
Jones E, Marsh S, O’Shaughnessy R, Aumailley M, McGrath JA, O’Toole EA and Caley MP (2022). 191 Junctional Epidermolysis Bullosa:Repairing The Epidermal Skin Barrier. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.202Hughes AJ, Thomas B, O’Toole EA and O’Shaughnessy R (2022). 183 Reduced FLG expression in atopic eczema reduces expression of key barrier genes and increases BMP signalling. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.194Wotherspoon DJ, Tachie-Menson T, Rogerson C and O’Shaughnessy R (2022). 174 Defining the composition and function of Lamin ribonuclear bodies during keratinocyte terminal differentiation and nuclear degradation. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.185Tawfik SS, Thomas B, Jones E, Caley MP, O’Shaughnessy R and O’Toole EA (2022). 144 Carbonylated proteins and Ozone in atopic eczema. Elsevier Journal of Investigative Dermatology vol. 142, (12) 10.1016/j.jid.2022.09.1542021
O’Shaughnessy RFL (2021). Understanding the paradoxical proinflammatory effects of an immunosuppressant. Oxford University Press (Oup) British Journal of Dermatology vol. 185, (6) 1091-1092. 10.1111/bjd.20778Wotherspoon DJ, Rogerson C and O’Shaughnessy R (2021). 111 Single cell RNA seq revealed altered cytoskeletal dynamics and the formation of cytoplasmic RNA-Lamin A bodies during keratinocyte terminal differentiation. Elsevier Journal of Investigative Dermatology vol. 141, (10) 10.1016/j.jid.2021.08.114Hughes AJ, Thomas B, O’Toole EA and O’Shaughnessy R (2021). 132 Increased BMP Signalling Correlates with Increased Eczema Severity. Elsevier Journal of Investigative Dermatology vol. 141, (10) 10.1016/j.jid.2021.08.135Button R, Harwood C and O’Shaughnessy R (2021). 282 Phosphoproteomic profiling of AKT-related changes in cutaneous squamous cell carcinoma identified DNA-dependent protein kinase (DNA-PK) and N-Myc downstream regulator 2 (NDRG2) as therapeutic vulnerabilities. Elsevier Journal of Investigative Dermatology vol. 141, (10) 10.1016/j.jid.2021.08.288O’Shaughnessy RFL (2021). Ceramide kinase‐like protein, ‘the kinase that isn’t’, finds a role in skin cancer stress relief. Oxford University Press (Oup) British Journal of Dermatology vol. 185, (1) 14-15. 10.1111/bjd.19888Wotherspoon D, Tommasi C, O'Shaughnessy R, Button R and Rogerson C (2021). Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation. Springer Nature [Academic Journals On Nature.Com] Cell Death and Differentiation 10.1038/s41418-020-00712-92020
Hughes AJ, Tawfik SS, Baruah KP, O'Toole EA and O'Shaughnessy RFL (2020). Tape Strips in Dermatology Research. Br J Dermatol 10.1111/bjd.19760O'Shaughnessy R, Rogerson C and Wotherspoon D (2020). Perspective: Controlling epidermal terminal differentiation with
transcriptional bursting and RNA bodies. Mdpi Ag Journal of Developmental Biology vol. 8, (4) 10.3390/jdb8040029O'Shaughnessy R (2020). Ubiquitin, a novel therapeutic target in psoriasis? Wiley British Journal of Dermatology 10.1111/bjd.193322019
O'Shaughnessy RFL (2019). Understanding actinic keratosis to reduce squamous cell carcinoma incidence. Wiley British Journal of Dermatology 10.1111/bjd.18529Tommasi C, Rogerson C, Depledge DP, Jones M, Naeem AS, Venturini C, Frampton D, Tutill HJ, Way B, Breuer J and O'Shaughnessy RFL (2019). Kallikrein-mediated cytokeratin 10 degradation is required for VZV propagation in skin. Elsevier Journal of Investigative Dermatology vol. 140, (Nat Biotech 34 2016) 774-784.e11. 10.1016/j.jid.2019.08.448O'Shaughnessy R and Papaioannou E (2019). Sonic Hedgehog-signalling limits chronic dermatitis via Gli2-driven immune regulatory function. American Society For Clinical Investigation Journal of Clinical Investigation 10.1172/JCI125170O'Shaughnessy RFL (2019). Targeting tryptophan transport and breakdown in basal cell carcinoma. Br J Dermatol vol. 180, (1) 16-17. 10.1111/bjd.173162018
O'SHAUGHNESSY RFL (2018). Differential expression of secreted factors SOSTDC1 and ADAMTS8 cause profibrotic changes in linear morphoea fibroblasts. Wiley British Journal of Dermatology 10.1111/bjd.17352Rogerson C and O'Shaughnessy RF (2018). Protein kinases involved in epidermal barrier formation: The AKT family and other animals. Exp Dermatol 10.1111/exd.13696Gutowska-Owsiak D, de La Serna JB, Fritzsche M, Naeem A, Podobas EI, Leeming M, Colin-York H, O’Shaughnessy R, Eggeling C and Ogg GS (2018). Orchestrated control of filaggrin–actin scaffolds underpins cornification. Springer Nature Cell Death & Disease vol. 9, (4) 10.1038/s41419-018-0407-22017
Rogerson C, Bergamaschi D and O'Shaughnessy RFL (2017). Uncovering mechanisms of nuclear degradation in keratinocytes: A paradigm for nuclear degradation in other tissues. Nucleus 1-9. 10.1080/19491034.2017.1412027Janus JM, O'Shaughnessy RFL, Harwood CA and Maffucci T (2017). Phosphoinositide 3-Kinase-Dependent Signalling Pathways in Cutaneous Squamous Cell Carcinomas. Mdpi Cancers vol. 9, (7) 10.3390/cancers9070086Naeem AS, Tommasi C, Cole C, Brown SJ, Zhu Y, Way B, Willis Owen SAG, Moffatt M, Cookson WO, Harper JI, Di WL, Reinheckel T and O'Shaughnessy RFL (2017). A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic dermatitis. Journal of Allergy and Clinical Immunology vol. 139, (4) 1228-1241. 10.1016/j.jaci.2016.09.052Duurland CL, Brown CC, O'Shaughnessy RFL and Wedderburn LR (2017). CD161+ Tconv and CD161+ treg share a transcriptional and functional phenotype despite limited overlap in TCRβ repertoire. Frontiers in Immunology vol. 8, (MAR) 10.3389/fimmu.2017.00103Zhu Y, Underwood J, Macmillan D, Shariff L, O'Shaughnessy R, Harper JI, Pickard C, Friedmann PS, Healy E and Di W-L (2017). Persistent kallikrein 5 activation induces atopic dermatitis-like skin architecture independent of PAR2 activity. Elsevier Journal of Allergy and Clinical Immunology vol. 140, (5) 1310-1322.e5. 10.1016/j.jaci.2017.01.0252016
Akinduro O, Sully K, Patel A, Robinson DJ, Chikh A, McPhail G, Braun KM, Philpott MP, Harwood CA, Byrne C, O'Shaughnessy RFL and Bergamaschi D (2016). Constitutive Autophagy and Nucleophagy during Epidermal Differentiation. Journal of Investigative Dermatology vol. 136, (7) 1460-1470. 10.1016/j.jid.2016.03.016Thomas AC, Zeng Z, Rivière J-B, O’Shaughnessy R, Al-Olabi L, St.-Onge J, Atherton DJ, Aubert H, Bagazgoitia L, Barbarot S, Bourrat E, Chiaverini C, Chong WK, Duffourd Y, Glover M, Groesser L, Hadj-Rabia S, Hamm H, Happle R, Mushtaq I, Lacour J-P, Waelchli R, Wobser M, Vabres P, Patton EE and Kinsler VA (2016). Mosaic Activating Mutations in GNA11 and GNAQ Are Associated with Phakomatosis Pigmentovascularis and Extensive Dermal Melanocytosis. Elsevier Journal of Investigative Dermatology vol. 136, (4) 770-778. 10.1016/j.jid.2015.11.0272015
Naeem AS, Zhu Y, Di WL, Marmiroli S and O'Shaughnessy RFL (2015). AKT1-mediated Lamin A/C degradation is required for nuclear degradation and normal epidermal terminal differentiation. Springer Nature Cell Death & Differentiation vol. 22, (12) 2123-2132. 10.1038/cdd.2015.62O'Shaughnessy RFL and Brown SJ (2015). Insight from the Air–Skin Interface. Elsevier Journal of Investigative Dermatology vol. 135, (2) 331-333. 10.1038/jid.2014.4572014
Kinsler VA, Drury S, Khan A, Waelchli R, Rukaite G, Barnicoat A, Lench N, Harper JI and O'Shaughnessy RFL (2014). A novel microdeletion in LOR causing autosomal dominant loricrin keratoderma. Oxford University Press (Oup) British Journal of Dermatology vol. 172, (1) 262-264. 10.1111/bjd.13361Duurland C, Brooks T, Hubank M, O'Shaughnessy R and Wedderburn L (2014). CD161+ regulatory t cells in immune regulation. Springer Nature Pediatric Rheumatology vol. 12, (Suppl 1) 10.1186/1546-0096-12-s1-p392013
Duurland C, Chain G, O'Shaughnessy R, Pesenacker A and Wedderburn L (2013). PReS-FINAL-1021: Further characterization of CD161+ regulatory t cells in health and disease. Springer Nature Pediatric Rheumatology vol. 11, (Suppl 2) 10.1186/1546-0096-11-s2-p17Gerner L, Youssef G and O'Shaughnessy RFL (2013). The protein phosphatase 2A regulatory subunit Ppp2r2a is required for Connexin‐43 dephosphorlyation during epidermal barrier acquisition. Wiley Experimental Dermatology vol. 22, (11) 754-756. 10.1111/exd.12234Youssef G, Ono M, Brown SJ, Kinsler VA, Sebire NJ, Harper JI and O'Shaughnessy RFL (2013). Identifying a Hyperkeratosis Signature in Autosomal Recessive Congenital Ichthyosis: Mdm2 Inhibition Prevents Hyperkeratosis in a Rat ARCI Model. Elsevier Journal of Investigative Dermatology vol. 134, (3) 858-861. 10.1038/jid.2013.374Youssef G, Gerner L, Naeem AS, Ralph O, Ono M, O’Neill CA and O’Shaughnessy RFL (2013). Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition. Elsevier Developmental Biology vol. 380, (2) 274-285. 10.1016/j.ydbio.2013.04.034Furmanski AL, O'Shaughnessy RFL, Saldana JI, Blundell MP, Thrasher AJ, Sebire NJ, Davies EG and Crompton T (2013). T-Cell Reconstitution after Thymus Xenotransplantation Induces Hair Depigmentation and Loss. Elsevier Journal of Investigative Dermatology vol. 133, (5) 1221-1230. 10.1038/jid.2012.4922012
O'Shaughnessy RFL, Chaudhury I and Harper JI (2012). Interleukin-1 alpha blockade prevents hyperkeratosis in an in vitro model of lamellar ichthyosis. Oxford University Press (Oup) Human Molecular Genetics vol. 22, (5) 1059-1059. 10.1093/hmg/dds511Sully K, Akinduro O, Philpott MP, Naeem AS, Harwood CA, Reeve VE, O'Shaughnessy RF and Byrne C (2012). The mTOR inhibitor rapamycin opposes carcinogenic changes to epidermal Akt1/PKBα isoform signaling. Springer Nature Oncogene vol. 32, (27) 3254-3262. 10.1038/onc.2012.338Ekeowa-Anderson AL, Purdie KJ, Gibbon K, Byrne CR, Arbeit JM, Harwood CA and O'Shaughnessy RFL (2012). AKT1 Loss Correlates with Episomal HPV16 in Vulval Intraepithelial Neoplasia. Public Library of Science (Plos) Plos One vol. 7, (6) 10.1371/journal.pone.00386082011
Jackson B, Brown SJ, Avilion AA, O'Shaughnessy RFL, Sully K, Akinduro O, Murphy M, Cleary ML and Byrne C (2011). TALE homeodomain proteins regulate site-specific terminal differentiation, LCE genes and epidermal barrier. Development vol. 138, (11) 1681-1690. Jackson B, Brown SJ, Avilion AA, O'Shaughnessy RFL, Sully K, Akinduro O, Murphy M, Cleary ML and Byrne C (2011). TALE homeodomain proteins regulate site-specific terminal differentiation, LCE genes and epidermal barrier. J Cell Sci vol. 124, (Pt 10) 1681-1690. 10.1242/jcs.0775522010
O'Shaughnessy RFL, Choudhary I and Harper JI (2010). Interleukin-1 alpha blockade prevents hyperkeratosis in an in vitro model of lamellar ichthyosis. Oxford University Press (Oup) Human Molecular Genetics vol. 19, (13) 2594-2605. 10.1093/hmg/ddq145Byrne C, Avilion AA, O'Shaughnessy RF, Welti JC and Hardman MJ (2010). Whole-mount assays for gene induction and barrier formation in the developing epidermis. Methods Mol Biol vol. 585, 271-286. 10.1007/978-1-60761-380-0_192009
O'Shaughnessy RFL, Welti JC, Sully K and Byrne C (2009). Akt-dependent Pp2a activity is required for epidermal barrier formation during late embryonic development. Development vol. 136, (20) 3423-3431. 10.1242/dev.0370102007
O'Shaughnessy RFL, Akgũl B, Storey A, Pfister H, Harwood CA and Byrne C (2007). Cutaneous human papillomaviruses down-regulate AKT1, whereas AKT2 up-regulation and activation associates with tumors. Cancer Res vol. 67, (17) 8207-8215. 10.1158/0008-5472.CAN-07-0755O'Shaughnessy RFL, Welti JC, Cooke JC, Avilion AA, Monks B, Birnbaum MJ and Byrne C (2007). AKT-dependent HspB1 (Hsp27) activity in epidermal differentiation. J Biol Chem vol. 282, (23) 17297-17305. 10.1074/jbc.M6103862002004
O'Shaughnessy RFL, Yeo W, Gautier J, Jahoda CAB and Christiano AM (2004). The WNT signalling modulator, Wise, is expressed in an interaction-dependent manner during hair-follicle cycling. J Invest Dermatol vol. 123, (4) 613-621. 10.1111/j.0022-202X.2004.23410.xO'Shaughnessy RFL, Christiano AM and Jahoda CAB (2004). The role of BMP signalling in the control of ID3 expression in the hair follicle. Wiley Experimental Dermatology vol. 13, (10) 621-629. 10.1111/j.0906-6705.2004.00206.xJahoda CAB, Kljuic A, O'Shaughnessy R, Crossley N, Whitehouse CJ, Robinson M, Reynolds AJ, Demarchez M, Porter RM, Shapiro L and Christiano AM (2004). The lanceolate hair rat phenotype results from a missense mutation in a calcium coordinating site of the desmoglein 4 gene. Elsevier Genomics vol. 83, (5) 747-756. 10.1016/j.ygeno.2003.11.015O'Shaughnessy RFL and Christiano AM (2004). Inherited disorders of the skin in human and mouse: from development to differentiation. Upv/Ehu Press The International Journal of Developmental Biology vol. 48, (2-3) 171-179. 10.1387/ijdb.152723822003
Kljuic A, Bazzi H, Sundberg JP, Martinez-Mir A, O'Shaughnessy R, Mahoney MG, Levy M, Montagutelli X, Ahmad W, Aita VM, Gordon D, Uitto J, Whiting D, Ott J, Fischer S, Gilliam TC, Jahoda CAB, Morris RJ, Panteleyev AA, Nguyen VT and Christiano AM (2003). Desmoglein 4 in Hair Follicle Differentiation and Epidermal Adhesion Evidence from Inherited Hypotrichosis and Acquired Pemphigus Vulgaris. Elsevier Cell vol. 113, (2) 249-260. 10.1016/s0092-8674(03)00273-32001
Aberger F, Costa-Pereira AP, Schlaak JF, Williams TM, O'Shaughnessy RFL, Hollaus G, Kerr IM and Frischauf A-MM (2001). Analysis of Gene Expression Using High-Density and IFN-γ-Specific Low-Density cDNA Arrays. Elsevier Genomics vol. 77, (1-2) 50-57. 10.1006/geno.2001.6623O’Shaughnessy RFL and Christiano AM (2001). Stem Cells in the Epidermis. Karger Publishers Skin Pharmacology and Physiology vol. 14, (6) 350-357. 10.1159/0000563682000
O'Shaughnessy RFL, Seery JP, Celis JE, Frischauf A-M and Watt FM (2000). PA‐FABP, a novel marker of human epidermal transit amplifying cells revealed by 2D protein gel electrophoresis and cDNA array hybridisation. Wiley Febs Letters vol. 486, (2) 149-154. 10.1016/s0014-5793(00)02252-3Grants
Defining the mechanism of skin barrier restoration by bacterial lysatesO'Shaughnessy RF and Coppock L
Biotechnology and Biological Sciences Research Council (25-09-2022 - 29-09-2026)
AKT-dependent control of barrier function and inflammation in eczemaO'Shaughnessy R
£236,971 Bart's Charity (01-07-2020 - 01-07-2023)
Dissecting actin mediated nuclear destruction during keratinocyte terminal differentiationBergamaschi D, O'Shaughnessy RF and Wotherspoon D
Biotechnology and Biological Sciences Research Council (23-09-2018 - 19-04-2023)
Finding new therapeutic avenues for ARCI by targeting common pathways of hyperkeratosisO'Shaughnessy RFL
£58,000 Foundation for Ichthyosis and Related Skin Types (01-09-2015 - 31-08-2021)
Identification of key AKT kinases and AKT targets in for prevention and treatment of squamous cell carciNOmaO'Shaughnessy R
£9,962 British Skin Foundation (31-07-2020 - 31-07-2021)
Understanding mechanisms of AKT expression and activity during skin cancer progressionO'Shaughnessy RFL
£194,013 Bart's Charity (01-04-2018 - 31-03-2021)
Understanding the Regulation of Epidermal Differentiation Genes by Filaggrin in EczemaO'Shaughnessy RFL and Aaron H
£265,041 Medical Research Council (01-02-2020 - 31-01-2021)
Establishment of the Livingstone skin research Centre in the Institute of Child HealthO'Shaughnessy RFL
£1,000,000 Great Ormond Street Hospital Charity (01-12-2017 - 01-12-2017)
Hedgehog signaling in atopic dermatitisO'Shaughnessy RFL
£70,000 Great Ormond Street Hospital Charity (01-10-2014 - 01-10-2017)
How do viruses infect, replicate and proliferate in skin?O'Shaughnessy RFL
£80,000 Great Ormond Street Hospital Charity (01-10-2014 - 01-10-2017)
Defining a common mechanism leading to hyperkeratosis in skin diseaseO'Shaughnessy RFL
£81,000 British Skin Foundation (01-01-2014 - 01-09-2017)
AKT1-Dependent HSPB1 phosphorlyation and filaggrin processing in atopic dermatitisO'Shaughnessy RFL
£75,000 British Skin Foundation (01-05-2010 - 01-05-2013)