Dr Clare Thompson

Clare ThompsonMBiochem, PhD

Emulate Centre Scientist

Centre for Predictive in vitro Models
Queen Mary University of London
+44 (0)20 7882 3603
ORCiD LinkedIn

Research

organ-on-a-chip, cartilage, synovium, arthritis, inflammation, cancer

Underpinning Bioengineering, Cancer Models, Musculoskeletal Models

Interests

As centre manager for the Queen Mary + Emulate Organ-on-Chips centre I have extensive expertise in the use of Emulates platform technology, The Human Emulation system across a range of organ models which include liver, lung, kidney and intestine. I provide support and advice for research teams that aspire to include this technology in their research pipeline. My research focuses on the importance of mechanical stimuli in the regulation of tissue interactions and cell signalling events. In particular, I have published extensively on the role of a small, microtubule-based signalling organelle, the primary cilium, demonstrating the importance of ciliary trafficking events for this regulation. I am currently developing new models in the rheumatology and oncology fields that incorporate physiologically relevant mechanical stimuli and with the ultimate goal of generating systems with greater capacity to predict the human condition. research proposals.

Publications

Publications of specific relevance to Predictive in vitro Models

2021

Verbruggen SW, Thompson CL, Duffy MP, Lunetto S, Nolan J, Pearce OMT, Jacobs CR and Knight MM (2021). Mechanical stimulation modulates osteocyte regulation of cancer cell phenotype. Cancers  vol. 13, (12) 10.3390/cancers13122906
Thompson C, Mcfie M, Chapple J, BEALES P and Knight M (2021). Polycystin-2 is required for chondrocyte mechanotransduction and traffics to the primary cilium in response to mechanical stimulation. Mdpi Ag  International Journal of Molecular Sciences  10.3390/ijms22094313
Wu H, Wang Z, Liu S, Meng H, Shelton JC, Thompson CL, Fu S and Knight MM (2021). Sub-toxic levels of cobalt ions impair mechanostranduction via HDAC6-depedent primary cilia shortening. Biochem Biophys Res Commun  10.1016/j.bbrc.2021.03.157
Thompson CL, Fu S, Heywood HK, Knight MM and Thorpe SD (2021). Corrigendum: Mechanical Stimulation: A Crucial Element of Organ-on-Chip Models (Front. Bioeng. Biotechnol., (2020), 8, 602646, 10.3389/fbioe.2020.602646). Frontiers in Bioengineering and Biotechnology  vol. 9, 10.3389/fbioe.2021.658873
Fu S, Meng H, Inamdar S, Das B, Gupta H, Wang W, Thompson C and Knight M (2021). Activation of TRPV4 by mechanical, osmotic or pharmaceutical stimulation is anti-inflammatory blocking IL-1β mediated articular cartilage matrix destruction., Editors: Block JA. Elsevier  Osteoarthritis and Cartilage  10.1016/j.joca.2020.08.002

2020

Thompson CL, Fu S, Knight MM and Thorpe SD (2020). Mechanical Stimulation: A Crucial Element of Organ-on-Chip Models. Frontiers Media  Frontiers in Bioengineering and Biotechnology  vol. 8, 10.3389/fbioe.2020.602646
Torres-Pérez JV, Naeem H, Thompson CL, Knight MM and Novak P (2020). Nanoscale mapping reveals functional differences in ion channels populating the membrane of primary cilia. Cellular Physiology and Biochemistry  vol. 54, (1) 15-26. 10.33594/000000202
Ahern DP, Fie MRM, Thompson CL, Duffy MP, Butler JS and Hoey DA (2020). Chapter 2.2 Primary Cilia Mechanobiology. Mechanobiology  10.1016/b978-0-12-817931-4.00006-6

2019

Fu S, Thompson CL, Ali A, Wang W, Chapple JP, Mitchison HM, Beales PL, Wann AKT and Knight MM (2019). Mechanical loading inhibits cartilage inflammatory signalling via an HDAC6 and IFT-dependent mechanism regulating primary cilia elongation. Osteoarthritis and Cartilage  vol. 27, (7) 1064-1074. 10.1016/j.joca.2019.03.003
Harris C, THORPE SD, Rushwan S, Wang W, THOMPSON C, Peacock J, KNIGHT M, Gooptu B and Greenough A (2019). An in vitro investigation of the inflammatory response to the strain amplitudes which occur during high frequency oscillation ventilation and conventional mechanical ventilation. Elsevier  Journal of Biomechanics  10.1016/j.jbiomech.2019.03.024
Ahern DP, Mc Fie MR, Thompson CL, Duffy MP, Butler JS and Hoey DA (2019). Primary cilia mechanobiology. Mechanobiology: From Molecular Sensing to Disease  10.1016/B978-0-12-817931-4.00006-6
O’Toole SM, Watson DS, Novoselova TV, Romano LEL, King PJ, Bradshaw TY, Thompson CL, Knight MM, Sharp TV, Barnes MR, Srirangalingam U, Drake WM and Chapple JP (2019). Oncometabolite induced primary cilia loss in pheochromocytoma. Endocrine-Related Cancer  vol. 26, (1) 165-180. 10.1530/ERC-18-0134

2017

Gambassi S, Geminiani M, Thorpe SD, Bernardini G, Millucci L, Braconi D, Orlandini M, Thompson CL, Petricci E, Manetti F, Taddei M, Knight MM and Santucci A (2017). Smoothened-antagonists reverse homogentisic acid-induced alterations of Hedgehog signaling and primary cilium length in alkaptonuria. Journal of Cellular Physiology  vol. 232, (11) 3103-3111. 10.1002/jcp.25761
Thompson CL, Plant JC, Wann AK, Bishop CL, Novak P, Mitchison HM, Beales PL, Chapple JP and Knight MM (2017). Chondrocyte expansion is associated with loss of primary cilia and disrupted hedgehog signalling. Eur Cell Mater  vol. 34, 128-141. 10.22203/eCM.v034a09
Schwarz N, Lane A, Jovanovic K, Parfitt DA, Aguila M, Thompson CL, da Cruz L, Coffey PJ, Chapple JP, Hardcastle AJ and Cheetham ME (2017). Arl3 and RP2 regulate the trafficking of ciliary tip kinesins. Oxford University Press  Hum Mol Genet  vol. 26, (13) 2480-2492. 10.1093/hmg/ddx143
Schwarz N, Lane A, Jovanovic K, Parfitt DA, Aguila M, Thompson CL, da Cruz L, Coffey PJ, Chapple JP, Hardcastle AJ and Cheetham ME (2017). Arl3 and RP2 regulate the trafficking of ciliary tip kinesins. (CORRIGENDUM). Oxford University Press  Hum Mol Genet  vol. 26, (17) 3451-3451. 10.1093/hmg/ddx245
THORPE SD, Gambassi S, Thompson CL, Chandrakumar C, Santucci A and Knight MM (2017). Reduced Primary Cilia Length and Altered Arl13b Expression Are Associated with Deregulated Chondrocyte Hedgehog Signalling in Alkaptonuria. John Wiley & Sons Inc.  Journal of Cellular Physiology  10.1002/jcp.25839

2016

KNIGHT MM, wang W, wang Z, wann A, thompson C and hassen A (2016). IFT88 influences chondrocyte actin organization and biomechanics. Osteoarthritis and Cartilage  10.1016/j.joca.2015.10.003
KNIGHT MM, Thompson CL, Wiles A and Poole CA (2016). Lithium chloride modulates chondrocyte primary cilia and inhibits hedgehog signalling. Federation of American Society of Experimental Biology (Faseb)  The Faseb Journal  10.1096/fj.15-274944
Sliogeryte K, Thorpe SD, Wang Z, Thompson CL, Gavara N and Knight MM (2016). Differential effects of LifeAct-GFP and actin-GFP on cell mechanics assessed using micropipette aspiration. Elsevier  Journal of Biomechanics  vol. 49, (2) 310-317. 10.1016/j.jbiomech.2015.12.034

2015

Thompson CL, Patel R, Kelly T-AN, Wann AKT, Hung CT, Chapple JP and Knight MM (2015). Hedgehog signalling does not stimulate cartilage catabolism and is inhibited by Interleukin-1β. Arthritis Research & Therapy  vol. 17, (1) 10.1186/s13075-015-0891-z
Thompson CL, Poole CA and Knight MM (2015). Lithium chloride modulates chondrocyte primary cilia and inhibits Hedgehog signaling. Federation of American Society of Experimental Biology  The Faseb Journal  vol. 100, (4) A39-A40. 10.1096/fj.15-274944
KNIGHT MM, Thompson CL, Yasmin H, Varone A, Wiles A and Poole CA (2015). Lithium chloride prevents interleukin- 1β induced cartilage degradation and loss of mechanical properties. Wiley: 12 Months  Journal of Orthopaedic Research  10.1002/jor.22913
Thompson CL, Poole CA and Knight MM (2015). Lithium chloride triggers primary cilia elongation and inhibits hedgehog signalling in articular chondrocytes. International Journal of Experimental Pathology  vol. 96, (2) A6-A6.
Thompson CL, Wann AKT, Chapple JP, Poole CA and Knight MM (2015). Competitive interactions between hedgehog and cytokine signalling: crosstalk at the chondrocyte primary cilium? International Journal of Experimental Pathology  vol. 96, (2) A8-A8.

2014

Thompson CL, Chapple JP and Knight MM (2014). Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes. Osteoarthritis Cartilage  vol. 22, (3) 490-498. 10.1016/j.joca.2013.12.016

2013

McMurray RJ, Wann AKT, Thompson CL, Connelly JT and Knight MM (2013). Surface topography regulates wnt signaling through control of primary cilia structure in mesenchymal stem cells. Sci Rep  vol. 3, 10.1038/srep03545
Wann AK, Thompson CL, Chapple JP and Knight MM (2013). Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium. Cilia  vol. 2, (1) 10.1186/2046-2530-2-17

2012

Thompson C, Prodromou N, Osborn D, Ashworth R, Knight M, Beales P and Chapple J (2012). Heat-shock induces rapid resorption of primary cilia. Cilia  vol. 1, (Suppl 1) p52-p52. 10.1186/2046-2530-1-s1-p52
Thompson C, Chapple J and Knight M (2012). Mechanical strain disrupts primary cilia structure and modulates hedgehog signalling in adult chondrocytes. Cilia  vol. 1, (Suppl 1) p53-p53. 10.1186/2046-2530-1-s1-p53
Prodromou NV, Thompson CL, Osborn DPS, Cogger KF, Ashworth R, Knight MM, Beales PL and Chapple JP (2012). Heat shock induces rapid resorption of primary cilia. J Cell Sci  vol. 125, (Pt 18) 4297-4305. 10.1242/jcs.100545
Wann AKT, Thompson C and Knight MM (2012). The role of the primary cilium in chondrocyte response to mechanical loading. Mechanically Gated Channels and Their Regulation  10.1007/978-94-007-5073-9_15