This webinar from the Preclinical Models section of the Orthopaedic Research Society presents an overview of animal-free in vitro preclinical systems including but not limited to 3D tissue cultures, organoids, and patient-derived explants. Including a talk from QMUL's CPM (Dr Stefaan Verbruggen), the audience will receive specific methods used in orthopaedic research, such as multi-physiological systems using chip technology to mimic bone, intervertebral disc, or cancer metastases. Through this scientific discussion, we aim to foster a broader adoption of these methodologies, thereby enhancing the rigor and ethical standards of preclinical research.